Cancer Cures

[The Irish Ram]

Stunning results from one drop:

 

[Mad Scientist]

I trust surgery not dope.

Surgery removes tumors but not the Cancer.

 

[badger2]

Yes, Parkinson's-Cannabis seems to have a different pharmacodynamic:

Parkinson's / Cannabis
Neurological Disorders in Medical Use Of Cannabis: an Update. - PubMed - NCBI

 

[badger2]

Since Zika is also a Flavivirus with neurological implications, we should begin to see more riboswitch applications for cancers and viruses. For HCV Flavivirus (note that the IRES also links to poliovirus):

IRES / Riboswitch / Hepatitis C Virus
Geneticin Stabilizes the Open Conformation of the 5' Region of Hepatitis C Virus RNA and Inhibits Viral Replication. - PubMed - NCBI

 

[badger2]

We would comment on the report "Neurological Disorders in Medical Use of Cannabis," except that it costs $95 to read. The book-burnings will continue to dumb down the prisoners in three dimensions, whilst business is good in two.

 

[badger2]

During the Zika months, we compared TWIST1 gene wityh Flavivirus-induced microcephaly, because of Drosophila null and mouse heterozygote null mutants, the Drosophila head twisted in the egg case. Not only does this link to Saethre-Chotzen syndrome, it links to breast cancer and melanoma.

Dec 2016 Breast Cancer / TWIST1
Mechanism of early dissemination and metastasis in Her2+ mammary cancer. - PubMed - NCBI

Sweden / Malanoma
Small RNA deep sequencing discriminates subsets of extracellular vesicles released by melanoma cells--Evidence of unique microRNA cargos. - PubMed - NCBI
'....Interestingly, miR-214 and miR-199a, both related to melanoma progression, are found in a cluster of miRNAs that are regulated by the transcription factor, TWIST1 which has been associated with neural cell development, which may be important, since melanocytes are known to originate from the neural crest.'

Note the coronal suture in Zika microcephaly from those reports, which compare to....

'....features are premature fusion of coronal sutures resulting in brachycephaly, low frontal hairline, , facial asymmetry, ptosis of the eyelids, prominent helical crus, syndactyly, and broad great toes.'
(Twist1 and the Saethre-Chotzen Syndrome, in Inborn Errors of Development:The Molecular Basis of Clinical Disorders of Morphogenesis)

 

[badger2]

Zika vector is female Aedes aegypti:

Sphaeropsidin / Aedes aegypti
Cyclopaldic acid, seiridin, and sphaeropsidin A as fungal phytotoxins, and larvicidal and biting deterrents against Aedes aegypti (Diptera: Culicid... - PubMed - NCBI
'....Sphaeropsidin B was the most active compound.'

 

[badger2]

TWIST1 Trajectories

Recall that CWD prions jumped across the Wisconsin River in 2012 and established itself in Davis County, Iowa. In post #164, we mentioned IRES and polio. In Hooper's The River: A Journey to the Source of HIV-AIDS, he devotes a chapter to Hilary Koprowski and the African polio vaccine program. Koprowski worked at Wistar, and died in 2013 near Philadelphia. Koprowski also attended a rabies seminar outside of Nairobi, and it is to proline that we link the rabiesvirus.

Therefore, the TWIST1 trajectories include this study:

TWIST1 / Proline
Destabilization of the TWIST1/E12 complex dimerization following the R154P point-mutation of TWIST1: an in silico approach. - PubMed - NCBI
'....dimer....'

Having noticed the dimer, we next investigated a broader view of proline at Pubmed, and made the Hsp70 connection:

Proline
The Effect of Selective D- or Nα-Methyl Arginine Substitution on the Activity of the Proline-Rich Antimicrobial Peptide, Chex1-Arg20. - PubMed - NCBI

Noticing the sidebar abstracts on the above page, we next linked proline-rich antimicrobial peptides to hsp70:

Anti-Hsp70 Selectivity
The antibacterial effect of a proline-rich antibacterial peptide A3-APO. - PubMed - NCBI
'....but not in mammaqlian hsp70....are discussed in detail.'

The problem we found is that it costs $58 to read the discussion.

But when we linked melanoma to hsp70, we made the prion connection, as we have already mentioned Denver industrial pollutioin as a source of CWD in Colorado deer, in this thread:

Melanoma / Hsp70
'Hsp70 is abundantly present in mitochondria of tumor cells....Wistar Institute, Philadelphia....'

Pubmed keyword search 'melanoma[AND]hsp70' also revealed the prion link:

Chile / Physodic Acid / Depsidones
Potential anticancer activity of lichen secondary metabolite physodic acid. - PubMed - NCBI
'....keywords: lichen compounds, depsidones, melanoma cells, apoptosis, Hsp70, reactive oxygen species.'

It is the depsidones from lichens that degrade prions.

 

[RodISHI]

TWIST1 Trajectories

Recall that CWD prions jumped across the Wisconsin River in 2012 and established itself in Davis County, Iowa. In post #164, we mentioned IRES and polio. In Hooper's The River: A Journey to the Source of HIV-AIDS, he devotes a chapter to Hilary Koprowski and the African polio vaccine program. Koprowski worked at Wistar, and died in 2013 near Philadelphia. Koprowski also attended a rabies seminar outside of Nairobi, and it is to proline that we link the rabiesvirus.

Therefore, the TWIST1 trajectories include this study:

TWIST1 / Proline
Destabilization of the TWIST1/E12 complex dimerization following the R154P point-mutation of TWIST1: an in silico approach. - PubMed - NCBI
'....dimer....'

Having noticed the dimer, we next investigated a broader view of proline at Pubmed, and made the Hsp70 connection:

Proline
The Effect of Selective D- or Nα-Methyl Arginine Substitution on the Activity of the Proline-Rich Antimicrobial Peptide, Chex1-Arg20. - PubMed - NCBI

Noticing the sidebar abstracts on the above page, we next linked proline-rich antimicrobial peptides to hsp70:

Anti-Hsp70 Selectivity
The antibacterial effect of a proline-rich antibacterial peptide A3-APO. - PubMed - NCBI
'....but not in mammaqlian hsp70....are discussed in detail.'

The problem we found is that it costs $58 to read the discussion.

But when we linked melanoma to hsp70, we made the prion connection, as we have already mentioned Denver industrial pollutioin as a source of CWD in Colorado deer, in this thread:

Melanoma / Hsp70
'Hsp70 is abundantly present in mitochondria of tumor cells....Wistar Institute, Philadelphia....'

Pubmed keyword search 'melanoma[AND]hsp70' also revealed the prion link:

Chile / Physodic Acid / Depsidones
Potential anticancer activity of lichen secondary metabolite physodic acid. - PubMed - NCBI
'....keywords: lichen compounds, depsidones, melanoma cells, apoptosis, Hsp70, reactive oxygen species.'

It is the depsidones from lichens that degrade prions.

Certain lichens are also used for bladder infections. Just a guess but I'd say that fungi are involved in those long term bladder infections too as there is a familiar scent that is reminiscent of rotten feet that comes with infections like baby dog had.

 

[RodISHI]

This is a pay for article but apparently some cancer patients can access it. Search phrases neuroblastoma and H4 neuroglioma cells. Not sure what the protocol for that would be. SETD8 Inhibition Activates p53 in High-Risk Neuroblastoma | Cancer Discovery . SETD8 Inhibition Activates p53 in High-Risk Neuroblastoma
DOI: 10.1158/2159-8290.CD-RW2017-013 Published March 2017


Lichen-derived compounds show potential for central nervous system therapeutics. - PubMed - NCBI Lichen; Neuro2A; Neurogenic activity; Neurosphere assay; Neurotrophic activity; Perlatolic acid

 

[badger2]

Tinea meets those qualifications, and we are getting closer to the Aedes aegypti link to freshwater marine fungi which would be proximal to Sphaeropsis along the Nile. To get a snapshot of the dying pines, look for small clusters of black pycnidia at the base of the needles. Using for bladder has to go further. What are the constituents?

 

[RodISHI]

I haven't checked the Usnea Lichen out that well. Thought maybe next order of herbs I may get some but not sure when that will be.
I haven't been out to the pine trees yet either as its been nasty weather. I have had questions about the lichen that grew on the fruit trees here but no one seemed to have answers for those questions either. I planted those fruit trees in 96-98 and by 2000 the bark was encased in colorful lichen that looks similar to that which grows on the rocks in Southern California that takes generations to grow.

 

[badger2]

TWIST1 Trajectories: The Mad Mink Connection

Tracking the occurrence of physodic acid, there is....

Physodic Acid / Breast Cancer
Cytotoxic activity of physodic acid and acetone extract from Hypogymnia physodes against breast cancer cell lines. - PubMed - NCBI

As with anti-cancer chemistry of taxol from the yew tree, we see anti-prion chemistry in Spruce:

Physodic Acid / Pice abies / Hypogemnia
Secondary metabolites of the lichen Hypogymnia physodes (L.) Nyl. and their presence in spruce (Picea abies (L.) H. Karst.) bark. - PubMed - NCBI

Obtaining the name of the lichen, we locate its occurrence precisely at Stetsonville, Taylor County, Wisconsin, location of mad mink disease outbreak due to the feeding of downed cattle to mink on a mink farm. The range map shows occurrence of H. physodes north of Stetsonville, though the mink farmer drove in a 50-mile radius to pick up the dead cows.

'Hypogymnia physodes (L.) Nyl. This species grows on bark, on twigs, of both conifers and deciduous woody plants, over mosses, and occasionally on soil or rock. It is circumpolar arctic to temperate. Contents: atranorin, physodic and 3-hydroxyphysodic acids, physodalic, conphysodalic and protocetraric acids plus an unknown.
(Thomson, JW, Lichens of Wisconsin)

 

[badger2]

Picea abies / Hypogymnia

But the chemistry is geographically deceptive, for the one Hypogymnia that occurs in the Apostle Islands is the one that links to Africa:

'Hypogymnia tubulosa (Schaerer) Hav.

This species grows on twigs and bark of conifers. It is circumboreal and in Africa. In North America it ranges in the northeast as far west as Minnesota, is in the Pacific states and the northern Rockies. In Wisconsin it is known only in the Apostle Islands.'
(Thomson, op cit)

 

[badger2]

TWIST1 Trajectories: Hypogymnia-Codonpocarpus Connections

We make the kuru connection with Hypogymnia lugubris, also occurring in South America and the Falklands:

Lichens of Australia
https://anbg.gov.au/abrs/lichenist/lichenchecklist_e_o.html
'Hypogymnia lugubris....New Guinea....'

The anti-prion depsidones may be occurring precisely in Fore country, epicenter of kuru, though we have no data on Aboriginal use of Hypogymnia even though it likely occurs on Codonocarpus, known and used by the Aborigines.

 

[badger2]

TWIST1 Trajectories: Pollution Monitoring

There is a glutamate-glutamic acid difference in the prion mutations of fallow deer vs. other deer. Denver industrial pollution for CWD in deer which were eaqting pine needles compares with Hypogymnia:

Scotland: Hypogymnia and Pollution
The low-technology monitoring of atmospheric metal pollution in central Scotland. - PubMed - NCBI

Slovakia: Hypogymnia and Pollution
Biomonitoring of heavy metals contamination by mosses and lichens around Slovinky tailing pond (Slovakia). - PubMed - NCBI

 

[RodISHI]

Tripped through the weeds, wet and bugs to get some Xanthoria lichen off the fruit trees and the Cotton wood trees (took a bit to for sure id the stuff and we have a lot of it on the trees but its pretty small stuff).

Antiproliferative, antibacterial and antifungal activity of the lichen Xanthoria parietina and its secondary metabolite parietin. ...................The extract inhibited proliferation and induced apoptosis, both effects being accompanied by modulation of expression of cell cycle regulating genes such as p16, p27, cyclin D1 and cyclin A. It also mediated apoptosis by activating extrinsic and intrinsic cell death pathways, modulating Tumor Necrosis Factor-related apoptosis-inducing ligand (TRAIL) and B-cell lymphoma 2 (Bcl-2), and inducing Bcl-2-associated agonist of cell death (BAD) phosphorylation. Our results indicate that Xanthoria parietina is a major potential source of antimicrobial and anticancer substances.......................Antiproliferative, antibacterial and antifungal activity of the lichen Xanthoria parietina and its secondary metabolite parietin. | GHL - Scientific and Technical Literature

 

[badger2]

Excellent, Rodishi! It's a matter of posting the actual structure of the molecule to this thread for comparisons with other compounds. Knowing the molecular weight of the p27 would help in comparison to the 27 kDa heat-shock protein linked to breast cancer. Xanthoria's photobiont is Trebouxia. Actually, Xanthoria parietina does not occur in Wisconsin, so it occurs in Iowa?

 

[RodISHI]

Yes its here but I cannot tell you exactly which exact of Xanthoria variety it is. I can mail you some if that would do you any good? There appears to be another type growing too which is different, not much of it though and its very tiny stuff.

 

[RodISHI]

Trebouxia would account for the times these lichen had a very green hue to them over the years.