Snake Meat......source of chinese virus

[badger2]

A nurse relates her COVID experience:

From the Frontlines

Gretchen “Awesome People Wear Masks” Winter, MDMay 24Well, friends, today was my last shift...

www.avianflutalk.com

'....Ladies and Gentlemen, I have had the flu, I've had mumps on the pancreas, chicken pox, measles, broken neck and had total knee replacement. If you were to combine the painful effects of all those into one, it would not touch the hell I experienced the last six days with this corona virus. There is no comparison. Nothing that I know of or have ever experienced compares to it.'

 

[beagle9]

A nurse relates her COVID experience:

From the Frontlines

Gretchen “Awesome People Wear Masks” Winter, MDMay 24Well, friends, today was my last shift...

www.avianflutalk.com

'....Ladies and Gentlemen, I have had the flu, I've had mumps on the pancreas, chicken pox, measles, broken neck and had total knee replacement. If you were to combine the painful effects of all those into one, it would not touch the hell I experienced the last six days with this corona virus. There is no comparison. Nothing that I know of or have ever experienced compares to it.'

Dude, are you in here carrying on a conversation with yourself ??

 

[badger2]

#742: You can hear Dr. Dena Grayson's COVID-19 conversation in her video and those of many other professionals by tuning into MedTwitter. A Med Twitter list is given by the nurse we have just mentioned, Tabitha111 (above post), and is on the Kazakhstan thread at Avian Flu Talk:

‘Unknown pneumonia’ deadlier than coronavirus

https://www.scmp.com/news/china/diplomacy/article/3092563/chinese-embassy-warns-deadly-unknown-pneumonia-kazakhstanThe...

www.avianflutalk.com

It's not good news.

 

[badger2]

History and biology of COVID-19 could take the same route as the embarrassing ebola story, with still no verified, documented host-reservoir in nature. This from Avian Flu Talk, is also reported at Promedmail, one minute ago:

Ebola Spreading

https://www.msn.en-ca/news/other/ebola-spreading-in-western-congo-with-nearly-50-confirmed-cases-who/ar-BB16HMRq?ocid=msedgntp

 

[badger2]

Source: Promedmail

https://www.mailchi.mp/a3622c52479b/ebola-situation-pidmiologique-du-12-juillet-2020

'....Bikoro....Kilamba....'

Bikoro is situated on the shore of Lake Tumba

https://www.maplandia.com/democratic-republic-congo/equateur/bikoro

 

[badger2]

We've already connected the COVID dots for dengue on this thread, and this should be a hotspot for treatment studies:

Florida Keys: Three More Dengue Cases

https://www.bradenton.com/latest-news/article244170267

 

[badger2]

https://www.bradenton.com/latest-news/article244170267.html

 

[badger2]

Here a difference is evident between chloroquine and hydroxychloroquine:

Chloroquine / Bipolar Disorder

Exacerbations of bipolar disorder triggered by chloroquine in systemic lupus erythematosus--a case report - PubMed

Despite precise definitions and exclusions for 19 syndromes of neuropsychiatric systemic lupus erythematosus (NPSLE), under some circumstances it appears to be difficult to differentiate whether neuropsychiatric symptoms are caused by SLE or by other reasons such as primary mental disorders or...

www.ncbi.nlm.nih.gov

 

[badger2]

Hydroxychloroquine inhibits dengue, Zika, ebola, rabies, influenza A & B, HSV-1, HIV-1, etc.

2015 Taiwan/Japan Hydroxychloroquine-Inhibited Dengue Virus is Associated with Host Defense Machinery

Hydroxychloroquine-inhibited dengue virus is associated with host defense machinery - PubMed

Hydroxychloroquine (HCQ) is an antimalarial drug also used in treating autoimmune diseases. Its antiviral activity was demonstrated in restricting HIV infection in vitro; however, the clinical implications remain controversial. Infection with dengue virus (DENV) is a global public health...

www.ncbi.nlm.nih.gov

Antimalarial Treatment May Have a Time-Dependent Effect on Lupus Survival: Data from a Multinational Latin American Inception Cohort

Antimalarial treatment may have a time-dependent effect on lupus survival: data from a multinational Latin American inception cohort - PubMed

Antimalarial drugs were shown to have a protective effect, possibly in a time-dependent manner, on SLE survival. These results suggest that the use of antimalarial treatment should be recommended for patients with lupus.

www.ncbi.nlm.nih.gov

'....seem to have a protective effect on survival, as noted in a study from Spain conducted in a homogeneous Caucasian population, in which a decrease in mortality was evident.'

 

[badger2]

40 minutes ago:

First COVID-19 Vaccine Tested in U.S. Poised for Final Testing

AOL - News, Politics, Sports & Latest Headlines

www.aol.com

 

[badger2]

Not much detail to this report:

Russia Accused of Hacking Vaccine

Coronavirus updates: UK, US, Canada accuse Russia of hacking virus vaccine trials. Get the latest here.

In other virus news: Georgia's governor voids mask orders in localities; GOP restricts attendance to Jacksonville convention; 1.3 million more jobless claims.

www.tucson.com

 

[badger2]

This is probably the world's leading candidate:

15 Jul 2020 Positive News on Oxford/AstraZeneca

Positive news on Oxford/AstraZeneca COVID-19 vaccine could come Thursday: report

Positive news on initial trials of the University of Oxford's potential COVID-19 vaccine that has been licensed to AstraZeneca could be announced as soon as Thursday, ITV's political editor Robert Peston said, citing a source. The potential vaccine is already in large-scale Phase III human...

www.news.yahoo.com

 

[badger2]

The Dutch have a unique situation for COVID-19 in mink:

16 Jul 2020 COVID-19-Infected Mink, The Netherlands

https://www.rijksoverheid.nl/actuee/nieuws/2020/07/15/nieuw-besmetting-covid-19-bij-nertsenbedrijf

'....The farm in Ledaecker includes 4500 mink dams. In total, 24 mink companies in The Netherlands have now been declared infected....The total adult mink population given was 2.32 million. This means that 20% of Dutch mink farms have already been infected....This event may lend the country's scientific community a unique opportunity to study the epidemiology of the virus in susceptible animal populations, in particular modes of spread, including aerogenic.'

 

[badger2]

And another 93,000 minks in Spain:

Promedmail: 16 Jul 2020 COVID-19 in Spanish Mink

Spain to cull 93,000 mink at a farm hit by coronavirus

Health authorities have ordered the culling of all 93,000 mink at a farm in eastern Spain to prevent human contagion after discovering that most of the animals there had been infected with the coronavirus.

www.reuters.com

13 Jul 2020 Obituary: Dr. Donalde Kaye, Promedmail Associate Editor

Donald Kaye, Infectious Disease News Editorial Board Member and mentor to many, dies at 88

Donald Kaye, MD, MACP, an influential infectious diseases clinician and researcher and longtime Infectious Disease News Editorial Board Member, died July 4 at the age of 88, his family announced.Kaye authored the informative and widely read “Eye on ID” column for Infectious Disease News since...

www.healio.com

'....While a faculty member at Cornell, he trained and influenced future luminaries in infectious diseases, including Gerald Mandell, Merle Sand, and Anthony Fauci.'

 

[badger2]

The report from Germany (post #391) shows TGEV coronavirus subsistence on steel surfaces at 20C for 28 days. We next link minks to TGEV:

Denmark / Mink TGEV

Coronavirus infection in mink (Mustela vison). Serological evidence of infection with a coronavirus related to transmissible gastroenteritis virus and porcine epidemic diarrhea virus - PubMed

Antibodies to a transmissible gastroenteritis virus (TGEV)-related coronavirus have been demonstrated in mink sera by indirect immunofluorescence, peroxidase-linked antibody assays and immunoblotting. This is the first serological evidence of a specific coronavirus infection in mink. The...

www.ncbi.nlm.nih.gov

ACE2 / Human Colon Epithelia / SARS-CoV / Mink Lung Epithelia

Exogenous ACE2 expression allows refractory cell lines to support severe acute respiratory syndrome coronavirus replication - PubMed

Of 30 cell lines and primary cells examined, productive severe acute respiratory syndrome coronavirus (Urbani strain) (SARS-CoV) infection after low-multiplicity inoculation was detected in only six: three African green monkey kidney epithelial cell lines (Vero, Vero E6, and MA104), a human...

www.ncbi.nlm.nih.gov

Are there msm followup reports for COVID-19 and salmon? This May 2020 report is for a salmon bafinivirus in goldfish, a member of the Coronaviridae:

Bacciliform Coronavirus / Chinook Salmon Bafinivirus (Nidovirus)

Isolation of a Chinook Salmon Bafinivirus (CSBV) in Imported Goldfish Carassius auratus L. in the United Kingdom and Evaluation of Its Virulence in Resident Fish Species - PubMed

This is the first record of a fish nidovirus isolated from a consignment of goldfish at the United Kingdom (UK) border. The full-length viral genome was 25,985 nt, sharing a 97.9% nucleotide identity with the Chinook salmon bafinivirus (CSBV) NIDO with two deletions of 537 and 480 nt on the ORF...

www.ncbi.nlm.nih.gov

'....ultrastructural observations in renal tubule epithelial cells of goldfish showed cylindrical tubes with external spike-like structures. This is the first record of a fish nidovirus isolated from a consignment of goldfish.'

We mentioned Nidoviruses in post #18 of this thread, in answer to post #17: Did the Coronavirus Start as a Bioweapon?

Did the coronavirus start as a bio weapon

Did the Chinese Coronavirus Start as a Bioweapon Program? | The China Report Chinese sure wanted this shut down

www.usmessageboard.com

Also mentioned via Gorbalenya and Koonin, here:

Snake Meat......source of chinese virus

This was posted @ AFT one minute ago: Totally Misery -- Scene from a Chinese Hospital Twitter

www.usmessageboard.com

Snake Meat......source of chinese virus

no it was from dog meat that had been bitten by a rabid skunk

www.usmessageboard.com

 

[badger2]

A Rab Trajectory

First, we link Dr. Zelenko's COVID-119 hydroxychloroquine treatment, which includes zinc, to lupus (also treated with hydroxychloroquine) and the Rab5- zinc finger assemblage:

Zinc Finger / Early Endosomal / Rab5

Endosomal localization of the autoantigen EEA1 is mediated by a zinc-binding FYVE finger - PubMed

EEA1, a 162-kDa autoantigen associated with subacute cutaneous systemic lupus erythematosus, is a coiled-coil protein localized to early endosomes and cytosol. At its C terminus, the protein contains a cysteine-rich motif, which is shared with Vps27, Fab1, and Vac1, yeast proteins implicated in...

www.ncbi.nlm.nih.gov

events of h
Pubmed has no entries for Rab5 and hydroxychloroquine. Next is shown the link to ZIKA and (chloroquine [italics]):

Mar 2020 ZIKV / Rab5 & 7 / Chloroquine (co-authored work from London, Beijing, State Key Laboratory, CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology);

Characterization of Zika Virus Endocytic Pathways in Human Glioblastoma Cells - PubMed

Zika virus (ZIKV) infections can cause microcephaly and neurological disorders. However, the early infection events of ZIKV in neural cells remain to be characterized. Here, by using a combination of pharmacological and molecular approaches and the human glioblastoma cell T98G as a model, we...

www.ncbi.nlm.nih.gov

'...Importantly, ZIKV infection was inhibited by silencing of Rab5 and Rab7....ZIKV likely moves to Rab5-positive early endosomes and Rab7-positive late endosomes before depositing its RNA into the cytoplasm. Our study for the first time, described the early infection events of ZIKV in human glioblastoma cell line T 98G....We first observed that ZIKV infection was inhibited by chloroquine & NH(4)Cl, indicating a requirement of low intracellular pH.'

We next link Rab to ACE for the Chinese Red Belly Toad, Bombina maxima:

Bradykinin-Arg-Arg / Rab / ACE

D-Arg0-Bradykinin-Arg-Arg, a Latent Vasoactive Bradykinin B2 Receptor Agonist Metabolically Activated by Carboxypeptidases - PubMed

We previously reported hypotensive and vasodilator effects from C-terminally extended bradykinin (BK) sequences that behave as B₂ receptor (B₂R) agonists activated by vascular or plasma peptidases. D-Arg⁰-BK-Arg-Arg (r-BK-RR) is a novel prodrug peptide...

www.ncbi.nlm.nih.gov

'....The amphibian bradykinin homologue, maximakinin....we previously described the brief hypotensive responses associated with tachycardia in response to the i.v. injection of increasingly low doses of bradykinin in anesthetized rats and the strong potentiation of the responses following pharmacologic ACE blockade....These results underscore the important role played by ACE, as the main r-BK-inactivating peptidase in the extracellular space....The active reaction product r-BK is itself cleared by ACE and in endosomes.'

 

[badger2]

A Mar 2017 report links Zika to frogs and armadillos:

Investigating the Potential Role of North American Animals as Hosts for Zika Virus - PubMed

The recent emergence of the mosquito-borne Zika virus (ZIKV) in the Americas has become a global public health concern. We describe a series of experimental infections designed to investigate whether animals within certain taxonomic groups in North America have the potential to serve as ZIKV...

www.ncbi.nlm.nih.gov

'....Infectious virus was isolated from blood only in frogs and armadillos; however, the magnitude of viremia was low. leopard frogs, garter snakes....neutralizing antibodies were detected after infection in goats, rabbits, ducks, frogs and pigs. This study indicates that the animals tested to date are unlikely to act as animal reservoirs for ZIKV, but that rabbits and pigs could potentially serve as sentinel species. Understan ding the transmission cycle and maintenance of ZIKV in animals will help in developing surveillance programs and preventive measures for future outbreaks.'

For bacilliform coronaviruses, there is

Grass Carp, Ctenopharyngodon

Identification and ultrastructural characterization of a novel virus from fish - PubMed

During routine investigations on fish, a virus (isolate DF 24/00) with novel morphological features and hitherto undescribed morphogenesis was isolated from a white bream (Blicca bjoerkna L.; Teleostei, order Cypriniformes). Cell-free virions consist of a rod-shaped nucleocapsid (120-150x19-22...

www.ncbi.nlm.nih.gov

Nidovirus-Associated Pneumonia in Python

Nidovirus-Associated Proliferative Pneumonia in the Green Tree Python (Morelia viridis) - PubMed

In 2014 we observed a noticeable increase in the number of sudden deaths among green tree pythons (<i>Morelia viridis</i>). Pathological examination revealed the accumulation of mucoid material within the airways and lungs in association with enlargement of the entire lung. We performed a full...

www.ncbi.nlm.nih.gov

Gill-Associated Virus (Nidovirales) of Shrimp

Gill-associated virus of Penaeus monodon prawns: an invertebrate virus with ORF1a and ORF1b genes related to arteri- and coronaviruses - PubMed

A 20089 nucleotide (nt) sequence was determined for the 5' end of the (+)-ssRNA genome of gill-associated virus (GAV), a yellow head-like virus infecting Penaeus monodon prawns. Clones were generated from a approximately 22 kb dsRNA purified from lymphoid organ total RNA of GAV-infected prawns...

www.ncbi.nlm.nih.gov

Australian Nidovirus of Snapping Turtle, Myuchelys

Identification of a novel nidovirus as a potential cause of large scale mortalities in the endangered Bellinger River snapping turtle (Myuchelys georgesi) - PubMed

In mid-February 2015, a large number of deaths were observed in the sole extant population of an endangered species of freshwater snapping turtle, Myuchelys georgesi, in a coastal river in New South Wales, Australia. Mortalities continued for approximately 7 weeks and affected mostly adult...

www.ncbi.nlm.nih.gov

'....Its closest relatives having been described in pythons and lizards....At necropsy, animals were in poor body condition, had bilateral swollen eyelids and some animals had tan foci on the skin of the ventral thighs. Histological examination revealed peri-orbital, splenic, and nephric inflammation and necrosis....Bellinger River Virus is the name proposed.''

 

[badger2]

Having linked Rab7 to Zika, comparable is the Rab7 passage in Epstein's Inborn Errors of Development: The Molecular Basis of Clinical Disorders of Morphogenesis, the link was to Charcot-Marie-Tooth disease:

'Mutations in the small GTPase Rab7 cause a peripheral sensory neuropathy called Charcot-Marie-Tooth disease type 2B (CMT2B). Rab7 regulates the recruitment of dynein/dynactin to endocytic compartments and trafficking in the late endocytic pathway. Importantly, Rab7 was also shown to control the trafficking and signaling of the nerve growth factor receptor, TrkA, suggesting that Rab7 defects may contribute to neurodegeneration by affecting transport of neurotrophins of their receptors. Another type of CMT2A, is due to a loss-of-function mutation in the motor domain of the plus-end directed microtubule motor KIF1Bbeta. Studies in heterozygous kif1B+/- mice revealed that reducing the gene dosage leads to impaired anterograde axonal transport of synaptic vesicle precursors. This reinforces the idea that disturbed axonal transport is a major problem in CMT2A.'

Thus, a Pubmed keyword search, 'coronavirus charcot-marie-tooth' yielded only one reference:

Aug 2020 COVID-19 / ALS / Charcot-Marie-Tooth

Amyotrophic lateral sclerosis care and research in the United States during the COVID-19 pandemic: Challenges and opportunities - PubMed

Coronavirus disease 2019 has created unprecedented challenges for amyotrophic lateral sclerosis (ALS) clinical care and research in the United States. Traditional evaluations for making an ALS diagnosis, measuring progression, and planning interventions rely on in-person visits that may now be...

www.ncbi.nlm.nih.gov

'Johns Hopkins, Columbia University Neurological Institute, Cedars-Sinai, Emory U., Washington University School of Medicine, Hershey Medical Center, Duke U., Barrow Neurological Institute.

Conflict of Interest Statement.....R.B. has consulted for Modulus Pharmaceuticals, Mitochondria in Motion, Acurastem, Sarepta....and the Charcot-Marie-Tooth Association.'

R.B. is Cedars-Sinai, Los Angeles.

 

[badger2]

The entries at Pubmed for the mRNA-1273 vaccine are not in chronological order. It is curious why Pubmed decides on this monkey-wrenching format only recently. We will arrange the five mRNA-1273 entries in a chron:

14 Jul 2020 Moderna Vaccine Phase I

Moderna Phase 1 results show coronavirus vaccine safe, induces immune response

Moderna Inc's <MRNA.O> experimental vaccine for COVID-19 showed it was safe and provoked immune responses in all 45 healthy volunteers in an ongoing early-stage study, U.S. researchers reported on Tuesday. No study volunteers experienced a serious side effect, but more than half reported mild...

www.news.yahoo.com

'...."If your vaccine can induce a response comparable with natural infection, that's a winner," Fauci said in a telephone interview.'

11 Jun 2020

SARS-CoV-2 mRNA Vaccine Development Enabled by Prototype Pathogen Preparedness - PubMed

A SARS-CoV-2 vaccine is needed to control the global COVID-19 public health crisis. Atomic-level structures directed the application of prefusion-stabilizing mutations that improved expression and immunogenicity of betacoronavirus spike proteins. Using this established immunogen design, the...

www.ncbi.nlm.nih.gov

5 May 2020

An Evidence Based Perspective on mRNA-SARS-CoV-2 Vaccine Development - PubMed

The first outbreak of coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) occurred in Wuhan, Hubei Province, China, in late 2019. The subsequent COVID-19 pandemic rapidly affected the health and economy of the world. The global approach...

www.ncbi.nlm.nih.gov

6 Ap 2020

A Comprehensive Literature Review on the Clinical Presentation, and Management of the Pandemic Coronavirus Disease 2019 (COVID-19) - PubMed

Coronavirus disease 2019 (COVID-19) is a declared global pandemic. There are multiple parameters of the clinical course and management of the COVID-19 that need optimization. A hindrance to this development is the vast amount of misinformation present due to scarcely sourced manuscript preprints...

www.ncbi.nlm.nih.gov

1 Ap 2020

[Progress and challenge of vaccine development against 2019-novel coronavirus (2019-nCoV)] - PubMed

The outbreak of 2019-novel coronavirus (2019-nCoV) infection poses a serious threat to global public health. Vaccination is an effective way to prevent the epidemic of the virus. 2019-nCoV along with severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome...

www.ncbi.nlm.nih.gov

We have already shown how amino acid sequences can be turned into music as a study aid in the Music Forum, Evolution of the Saxophone. Arrange piano keys (for instance) as we have done for sax. See chart in post #2. Now the reader can listen to the music at precisely the point targeted by the vaccine: position 1273 of the COVID-19 spike protein:

Scroll down to sequences:

UniProt

www.uniprot.org

'....SEPVLKGVKL HYT....'

The threonine is position 1273.

 

[badger2]

This from Avian Flu Talk, antibody decline is an important issue:

Studies show coronavirus antibodies may fade fast, raising questions about vaccines

Recent studies indicate that the human body does not retain the antibodies that build up...

www.sfchronicle.com