Snake Meat......source of chinese virus

[Godboy]

What's fascinating is that you haven't the stones to reply objectively to any of the material we have given the readers.

You think you have readers?

I just read his posts, so yeah. We also know for a fact that you have no objective reply.

 

[Flash]

My god, snake meat?

What the hell are those people on Naked and Afraid going to eat?

 

[Flash]

I wonder if American Moon Bats contracted the Trump Derangement Syndrome mental Illness by eating snake meat?

 

[badger2]

Our "replying to oneself" was actually to get the info onto the net. Now, due to the plethora of COVID-19 threads, we can direct people to this USMB thread for further reading of the material, especially the report from Wuhan/Los/Alamos/U of C for potential reservoirs in nature, Chrysemys and Pelodiscus. This thread will remain objective and scientific while citing the authors, regardless of other reactionary postings.

 

[badger2]

In this article, readers can see the top antiviral compound docking in the COVID-19 pocket:

Rapid Identification of Potential Inhibitors of SARS-CoV-2 Main Protease by Deep Docking of 1.3 Billion Compounds
online.wiliey.com/doi/abs/10.1--2/minf.2000028
'Fig. 3 a) Predicted Binding Pose of our top hit compound (shown in magenta sticks) in the SARS-CoV-2 pocket....Our DD screening identified 585 new scaffolds for SARS-CoV-2 that are not shared with known protease inhibitors, although they can establish all the critical interactions with the protease active site, thus providing a completely new set of chemicals for testing and optimization.'

Composition and divergence of coronavirus spike proteins and host ACE2 receptors predict potential intermediate hosts of SARS-CoV-2. - PubMed - NCBI
'Other than pangolins and snakes, as previously suggested, turtles (Chrysemys picta bellii, Chelonia mydas, and Pelodiscus sinensis) may act as potential intermediate hosts transmitting SARS-CoV-2 to humans.'

 

[badger2]

Deaths: 7,140 (post #279).

 

[Unkotare]

Somebody thinks he’s important!

 

[badger2]

We think that the antiviral trajectory is important one to follow in conjunction with the vaccine trajectory, the latter taking more time to FDA approval. By including important genetic links to potential reservoirs and vectors of COVID-19 especially inside North America, insight into Thai use of HIV antiviral therapy will link to post # 605 for the docking of COVID19 protease inhibitors.

 

[badger2]

We also wish to invite new visitors to USMB and to this thread, as well as the Music Forum thread, Evolution of the Saxophone, where readers can hear the sound of the COVID-19 genome (if they accept the science-based protocol on which that amino acid music is based).

 

[badger2]

The latest cases in Beijing implicate pangolins.

Home - ProMED - ProMED-mail

ProMED is the largest publicly-available surveillance system conducting global reporting of infectious diseases outbreaks. Subscribe today.

promedmail.org

'....15 Jun 2020 'Our preliminary assessment is the virus came from overseas. We still can't determine how it got here. It might've been on contaminated seafood or meat, or spread from the faeces of people inside the market," state media quoted Yang Peng as saying. PromedMail would like to thank Daniel Lucy, M.D., for alerting us to this outbreak yesterday (13 Jun 2020). Media reports at the time mentioned that positive environmental samples included cutting boards used for cutting imported salmon from Europe (reportedly from Norway and Denmark's Faroe Islands)....Other media reports have mentioned that pangolins were sold in the market, as one of the suspected intermediate hosts (but nor proven) and how the Jingshen Seafood Market has a history of selling pangolins. Submitted and translated by ProMed rapporteur Dan Silver

https://www.chuannane.com/shiyao/20170215/7029.html

....'

 

[White 6]

These idiots really need to do something about there damned diet!

 

[badger2]

Recent Developments in Chinese Culinary Promiscuity

Should the World Be Worried?

Should the World Be Worried About the 'Explosive' New Outbreak of Coronavirus in Beijing?

Since 106 new cases emerged around a food market, the Chinese capital has been plunged into what officials are calling “wartime mode.”

www.yahoo.com

 

[badger2]

Here we attempt to reinforce the potential COVID-19 seafood link to dexamethasone:

Dexamethasone Hyperresponsiveness / Parainfluenza Virus

Dexamethasone prevents virus-induced hyperresponsiveness via multiple mechanisms - PubMed

In the lungs, neuronal M2 muscarinic receptors inhibit acetylcholine release from the parasympathetic nerves. Parainfluenza virus infection causes loss of M2 receptor function, which increases acetylcholine release and vagally mediated bronchoconstriction. Because glucocorticoids are known to...

www.ncbi.nlm.nih.gov

A potential investigative trajectory is parainfluenza virus-infected sea lice of oarfish.

Next we bring up salmon androsterone due to the cutting boards being contaminated:

Identification and distribution of the Leydig cell homolog in the testis of sexually mature Atlantic salmon (Salmo salar) - PubMed

Identification and distribution of the Leydig cell homolog in the testis of sexually mature Atlantic salmon (Salmo salar)

www.ncbi.nlm.nih.gov

A functional and structural androsterone analogue is methylcyclohexanone, which is a pheromone of the most destructive forest insect in North America, the bark beetle, Dendroctonus ponderosae. Next we link two viruses to cyclohexanone. One of them is the respiratory link, the other is the link to Thai anti-HIV meds.

Cyclohexanone Newcastle Disease Virus

Biologically active polycycloalkanes. 1. Antiviral adamantane derivatives - PubMed

Convenient methods for the synthesis of 1-substituted 3-adamantyl chlorides and bromides (2), 1-adamantylphenols and -cresols (3), and 1-adamantylacetic (6) as well as 1,3-adamantanediacetic (11) acids are described. Several novel derivatives were synthesized from these key intermediates...

www.ncbi.nlm.nih.gov

Cyclohexanone HIV-1 Integrase

2,6-Bis(3,4,5-trihydroxybenzylydene) derivatives of cyclohexanone: novel potent HIV-1 integrase inhibitors that prevent HIV-1 multiplication in cell-based assays - PubMed

A number of 2,6-bisbenzylidenecyclohexane-1-one derivatives have been synthesized and tested as HIV-1 integrase (IN) inhibitors with the aim of obtaining compounds capable to elicit antiviral activity at non-cytotoxic concentrations in cell-based assays...

www.ncbi.nlm.nih.gov

Next we include a porcine link, recalling the German study posted in this thread that showed a porcine intestinal coronavirus subsisting on steel surfaces for up to 24 days:

Pig Testicular 20beta-hydroxysteroid Dehydrogenase / 2-Methylcyclohexanone

Carbonyl reductase activity exhibited by pig testicular 20 beta-hydroxysteroid dehydrogenase - PubMed

The carbonyl reductase activity exhibited by pig testicular 20 beta-hydroxysteroid dehydrogenase (20 beta-HSD) was examined using a recombinant enzyme. Kinetic parameters were obtained for 48 carbonyl group-containing substrates, including aromatic aldehydes, aromatic ketones, cycloketones...

www.ncbi.nlm.nih.gov

 

[badger2]

USMB British thread for COVID-19/dexamethasone does not mention that Newcastle virus came from Newcastle-upon-Tyne in 1927.

Virulent Newcastle Disease

Virulent Newcastle disease - Wikipedia

en.wikipedia.org

Isopeptidases are a class of cysteine proteases, the latter having already been mentioned in this thread. Here we link cyclohexanone to isopeptidase inhibition:

Feb 2018 Isopeptidase Inhibition Based on the Bis (Arylidene) Cyclohexanone Scaffold

The binding landscape of a partially-selective isopeptidase inhibitor with potent pro-death activity, based on the bis(arylidene)cyclohexanone scaffold - PubMed

Diaryldienone derivatives with accessible β-carbons show strong anti-neoplastic properties, related to their ability to make covalent adducts with free thiols by Michael addition, and low toxicity in vivo. Accumulation of poly-ubiquitylated proteins, activation of the unfolded protein response...

www.ncbi.nlm.nih.gov

 

[badger2]

At thePubmed page for the "Binding Landscape" article above, there is a section titled "Similar Articles." The first entry is "Synthesis, Characterization, and Optimization for In Vivo Delivery of a Nonselective Isopeptidase Inhibitor As a New Antineoplastic Agent."

Synthesis, characterization, and optimization for in vivo delivery of a nonselective isopeptidase inhibitor as new antineoplastic agent - PubMed

Bis-arylidenecycloalkanones structurally related to the nonselective isopeptidase inhibitor G5 were synthesized and tested for cytotoxic activity against glioblastoma cells. Cytotoxicities correlate well with Hammett σ constants for substituted arylidene groups, confirming the proposed...

www.ncbi.nlm.nih.gov

Therefore, the pig/bark beetle assemblage is on a correct investigative trajectory for COVID-19.

Jun 2020 Dexamethasone / COVID-19 / Oncology

Routine antiemetic prophylaxis with dexamethasone during COVID-19: Should oncologists reconsider? - PubMed

The ongoing pandemic caused by severe acute respiratory syndrome (SARS) coronavirus type 2 (SARS-CoV-2, also known as COVID-19) has caused unprecedented strain on the global healthcare system, causing thousands of deaths worldwide. Patients with underlying conditions such as cancer are at...

www.ncbi.nlm.nih.gov

 

[CrusaderFrank]

Thanks, Obama!

 

[badger2]

The Beijing cutting board contamination was from salmon of Norway, Denmark or the Faroe Islands.

Sea Lice / Salmon Farms, Faroe Islands

Statistical modelling of sea lice count data from salmon farms in the Faroe Islands - PubMed

Fiskaaling regularly counts the number of sea lice in the attached development stages (chalimus, mobiles and adult) for the salmon farms in the Faroe Islands. A statistical model of the data is developed. In the model, the sea-lice infection is represented by the chalimus (or mobile) lice...

www.ncbi.nlm.nih.gov

Jan 2019 Lepeophtheirus salmonis (Kroeyer 1837) / Infectious Salmon Anemia Virus

Sea lice, Lepeophtheirus salmonis (Krøyer 1837), infected Atlantic salmon (Salmo salar L.) are more susceptible to infectious salmon anemia virus - PubMed

The role of parasitic sea lice (Siphonostomatoida; Caligidae), especially Lepeophtheirus salmonis, in the epidemiology of Infectious Salmon Anemia Virus (ISAv) has long been suspected. The epidemiological studies conducted during the 1998 major Infectious Salmon Anaemia (ISA) outbreak in...

www.ncbi.nlm.nih.gov

Salmon Lice / IHNV

Vector potential of the salmon louse Lepeophtheirus salmonis in the transmission of infectious haematopoietic necrosis virus (IHNV) - PubMed

To better understand the role of vector transmission of aquatic viruses, we established an in vivo virus-parasite challenge specifically to address (1) whether Lepeophtheirus salmonis can acquire infectious haematopoietic necrosis virus (IHNV) after water bath exposure or via parasitizing...

www.ncbi.nlm.nih.gov

'....mechanical rather than a biological vector or reservoir.'

Dexamethasone / Atlantic Salmon

Divergent regulation of insulin-like growth factor binding protein genes in cultured Atlantic salmon myotubes under different models of catabolism and anabolism - PubMed

Much attention has been given to insulin-like growth factor (Igf) pathways that regulate the balance of skeletal muscle protein synthesis and breakdown in response to a range of extrinsic and intrinsic signals. However, we have a less complete understanding of how the same signals modulate...

www.ncbi.nlm.nih.gov

Dexamethasone / Salmo / Gyrodactylus

Dexamethasone treatment affects skin mucous cell density in Gyrodactylus derjavini infected Salmo salar - PubMed

Atlantic salmon, Salmo salar, is normally rather refractive to infection with the ectoparasitic monogenean Gyrodactylus derjavini but dexamethasone treatment of the host increases the susceptibility. The causative mechanisms were elucidated in this work. Groups of Atlantic salmon were treated by...

www.ncbi.nlm.nih.gov

'....mucous cell discharge elicited by infection is inhibited by the drug.'

Dexamethasone / Myeloma

Effects of lenalidomide and dexamethasone treatment duration on survival in patients with relapsed or refractory multiple myeloma treated with lenalidomide and dexamethasone - PubMed

Continued lenalidomide treatment until disease progression after achievement of ≥PR is associated with a significant survival advantage when controlling for patient characteristics. These findings should be confirmed in a prospectively designed trial.

www.ncbi.nlm.nih.gov

Covid-19 / Myeloma

First case of COVID-19 in a patient with multiple myeloma successfully treated with tocilizumab - PubMed

<ol><li><p>We report the first case of coronavirus disease 2019 (COVID-19) in a multiple myeloma patient successfully treated with tocilizumab.</p></li><li><p>Although tocilizumab was effective in the treatment of COVID-19 in this case, randomized controlled trials are n</p></li></ol> …

www.ncbi.nlm.nih.gov

 

[badger2]

PubMed shows 3 entries for coronavirus and isopeptidase, we have already mentioned mouse hepatitis virus early on in the thread.

MHV

X-ray Structural and Functional Studies of the Three Tandemly Linked Domains of Non-structural Protein 3 (nsp3) from Murine Hepatitis Virus Reveal Conserved Functions - PubMed

Murine hepatitis virus (MHV) has long served as a model system for the study of coronaviruses. Non-structural protein 3 (nsp3) is the largest nsp in the coronavirus genome, and it contains multiple functional domains that are required for coronavirus replication. Despite the numerous functional...

www.ncbi.nlm.nih.gov

A noncovalent class of papain-like protease/deubiquitinase inhibitors blocks SARS virus replication - PubMed

We report the discovery and optimization of a potent inhibitor against the papain-like protease (PLpro) from the coronavirus that causes severe acute respiratory syndrome (SARS-CoV). This unique protease is not only responsible for processing the viral polyprotein into its functional units but...

www.ncbi.nlm.nih.gov

Characterization of ubiquitin and ubiquitin-like-protein isopeptidase activities - PubMed

Conjugation or deconjugation of ubiquitin (Ub) or ubiquitin-like proteins (UBLs) to or from cellular proteins is a multifaceted and universal means of regulating cellular physiology, controlling the lifetime, localization, and activity of many critical proteins. Deconjugation of Ub or UBL from...

www.ncbi.nlm.nih.gov

 

[badger2]

AsiaOne states that more than 1200 flights have been cancelled. Another headline states "Coronavirus: China Halts Salmon Imports Over Possible Link to Beijing Outbreak."

17 Jun 2020 Danish Mink Face Cull After Catching Coronavirus

https://www.thelocal.dk/20200617/danish-mink-face-slaughter-after-catching-coronavirus

 

[badger2]

The isopeptidase article of post #614 also states: 'UB or UBL fused to the reporter enzyme phospholipase A(2) (pla(2))….ubiquitin-like protein isopeptidase activities.'

PubMed search 'covid-19 phospholipase A2' yields no references, 'covid-19 phospholipase' makes the chloroquine connection;

May 2020 Chloroquine / Phospholipase

The endosomal lipid bis(monoacylglycero) phosphate as a potential key player in the mechanism of action of chloroquine against SARS-COV-2 and other enveloped viruses hijacking the endocytic pathway - PubMed

The anti-malarial drug Chloroquine (CQ) and its derivative hydroxychloroquine have shown antiviral activities in vitro against many viruses, including coronaviruses, dengue virus and the biosafety level 4 Nipah and Hendra paramyxoviruses. The in vivo efficacy of CQ in the treatment of COVID-19...

www.ncbi.nlm.nih.gov