COVID-19’s Biological Politics

[badger2]

The report in post #1,260 mentions similarity of SARS-CoV-2 to human ENaC.

2016 Israel / Human ENaC

Epithelial sodium channel (ENaC) family: Phylogeny, structure-function, tissue distribution, and associated inherited diseases - PubMed

The epithelial sodium channel (ENaC) is composed of three homologous subunits and allows the flow of Na(+) ions across high resistance epithelia, maintaining body salt and water homeostasis. ENaC dependent reabsorption of Na(+) in the kidney tubules regulates extracellular fluid (ECF) volume and...

pubmed.ncbi.nlm.nih.gov

'....Keywords: Renin-Angiotensin-Aldosterone system.'

We linked the aorta to this system in post #62 of this thread:

RN of 17 years tells you whats really going on in hospitals!

How so? (I could never be bothered to read the Harry Potter stuff.) purebloods, half bloods, mudbloods.

www.usmessageboard.com

'(Springer Protocols: The Renin-Angiotensin-Aldosterone System).'

The trajectory to the aorta/RAA system was precisely influenced by noticing Quay's Twitter-published sequence for SL-ZC45 coronavirus rgd motif that came just before the deletion: RGDP.

 

[ninja007]

the so called covid scam virus hasnt been id'd yet in the wild; its not real; does not exist.

 

[badger2]

Quay has explained the Bruttel, Washburne and VanDongen paper:

https://twitter.com/Quay_dr

 

[badger2]

The Debates Begin

https://twitter.com/julienpotet

 

[badger2]

When one begins to follow the Andersen thread, it will link to Jeremy Farrar. This is the JFK Jr. link in his book, The Real Anthony Fauci.

 

[badger2]

Calling Andersen out, it's @1GenieInABottle 4h who opens the trajectory back to Kennedy for the British MI6 passage. We are now in the realm of CIA-MI6 disinformation internet propaganda.

 

[badger2]

Ebright shows that indeed, the new Boston University virus used the Omicron spike:

https://twitter.com/R_H_Ebright

Because of the Omicron N969K mutation in the heptad repeat region (HR1&2), an examination of this SARS-CoV-1 report from 2005 shows where (either the virus or humans) got the idea to mutate from N to K.

Clicking retrieves the article, and scrolling down to Fig 1 we clearly see the lysines (K) in bold as well as an N (asparagine) in the sequence. Following the arrows from the boldened K's shows clues to what badger calls a coronavirus modus operandi in the use of branched-chain amino acids (isoleucine (I), leucine (L and valine (V), the latter three branched-chain aminos already mentioned in this thread.

Note that the location is also Boston, and since Omicron N969K can be included in the parameters of this 2005 work which was experimenting with increased-decreased fusion capabilities, heptad repeat region also links to vaccine production and protein stability:

Oct 2005 Harvard Medical School, Boston; Brigham and Women's Hospital, Boston; Louisiana State University, Baton Rouge

Genetic analysis of the SARS-coronavirus spike glycoprotein functional domains involved in cell-surface expression and cell-to-cell fusion - PubMed

The SARS-coronavirus (SARS-CoV) is the etiological agent of severe acute respiratory syndrome (SARS). The SARS-CoV spike (S) glycoprotein mediates membrane fusion events during virus entry and virus-induced cell-to-cell fusion. To delineate functional domains of the SARS-CoV S glycoprotein...

pubmed.ncbi.nlm.nih.gov

'....Specifically to investigate the amino acid requirements of the HR1 (heptad repeat region 1) of the SARS-CoV S glycoprotein, the a and d amino acid positions L [898] and N [901] were both replaced by lysine (K) residues in the cluster mutation CL2, effectively collapsing a predicted alpha-helical structure at the amino acid terminal terminus of the HR.

The CL5 cluster-to-lysine mutation was placed adjacent to the HR1 region.'

Omicron N969K is the penultimate amino acid position to the end of HR1, so this 2005 placement of lysine cluster mutations in proximity to HR1, as well as the lysines within it, is strong evidence that the Boston University scientists were not unaware of this previous work before they recombined Omicron with the ancestral strain.

 

[badger2]

Demaneuf goes into detail about the Baric virus (SHC014) and mentions Gao and the claimed HIV inserts. However, Demaneuf fails to record the uncultured marine virus we have already compared to the HIV inserts here at USMB (search: 'uncultured marine virus').

Timepoint 16h, that is the thread for Baric and the HIV inserts:

https://twitter.com/gdemaneuf

'@16h: Just reminding everyone about the incredible level of unethical behaviour, manipulations and shenanigans that lay behind some of the most prominent papers denying a labo origin. Show Thread.'

 

[badger2]

UNC Chapel Hill lab is mentioned at 10h:

https://twitter.com/thackerpd

 

[badger2]

From Alex Washburne's page, this is the cutting-edge paper of interest for SARS-CoV-2 origins, whether lab or nature:

https://www.anandamide.substack.com/p/a-deep-dive-on-typeiis-restriction

 

[badger2]

A deep dive on TypeIIs Restriction enzymes

And why SARs-CoV-2 is Lab derived

anandamide.substack.com

 

[badger2]

As the report in post # 1,271 states: 'ZC45.' We have already posted on ZC45 for the RGDP sequence. This paper also links our previous posts in this thread for heptad repeat region: N, K, N969K, etc. : 'N--->K from Wuhan1 to Omicron.'

The paper continues:

'Once Baric decides to put the GFPs (green fluorescent proteins) in frame of a gene, he can't introduce a new TypeIIs without changing the protein, so he resorts to No-See-Ums approach for this piece. Tops, restriction map of SARS-CoV-2 and the Bottom restriction map is of Baric's synthetic construct with GFP added.

No new restriction sites despite the addition of GFP.... So a paper submitted in Ap 2020 from Baric's lab constructs SARS-CoV-2 synthetically, very similar to as Bruttel et al predict.

If you are familiar with the complexity and scope of this paper, I'm very skeptical this started after the pandemic. There is at least a year's worth of work in this paper submitted in Ap 2020. It was being worked on before the pandemic emerged.'

 

[badger2]

Quay mentions Mengla, shows a map, and speaks of bat caves, though this is far from the location of Daszak's RsSHC014, collected in 2011:

https://twitter.com/Quay_dr

RsSHC014 came from the southern shore of Dian Lake.

Dian Lake - Wikipedia

en.wikipedia.org

Dian Lake, along the expressway that also connects the Mojiang copper mine (nearer to Mengla) is an intriguing location, not far from the nasty flower and animal market at Kunming, where everything is sold, from jungle orchids to tropical snakes.

USMB search:

1. Dian Lake
2. Kunming

 

[badger2]

At 15h, Demaneuf shows....

https://twitter.com/gdemaneuf

'....GOPHELP concludes a lab-leak is the most likely origin of pandemic....coming days before the election - lays out a template for possible GOP probes in next congress.'

 

[badger2]

We've already mentioned the N (asparagine) and K (lysine) links to Omicron and the Boston University study for the heptad repeat (post # 1, 267), as well as the RGD motif of bat virus ZC45.

Post # 1,273 mentions Mengla, and here there is a Marburg link to the Mengla filovirus and C-type lectins. This is the RGD motif link we see in the AC45 bat virus.

We now go back to the highly controversial article, rejected by Nature magazine, recallint that both lysine and glutamic acid are hydrophilic, charged amino acids:
Rejected by Nature Magazine

The fight for a controversial article

Birger Sørensen and Angus Dalgleish failed to get an article about the origins of the coronavirus published in a scientific journal. The authors suspect foul play and political considerations. Not everything gets published, is the answer from the journals. Minerva has obtained a draft of the...

www.minervanett.no

'....This additional lysine (K) driven charge on SARS-CoV-2 coming from the domain 526-560 does not exist on SARS-CoV due to the unique SARS-CoV-2 Cys538-Cys590 bridge.
....
Ref: Marzi A, et al (2004) "DC-SIGNR" Interact with the Glycoprotein of Marburg Virus and the S Protein of SARS-CoV.'

The Marzi et al study links the RGDP motif first noticed in ZC45 on Dr. Quay's Twitter page.

We can now link the C-type lectins to ebola, Marburg and Mengla filoviruses, though the Sorensen-Dalgleish paper does not address the heptad repeat regions when they find unusual electrical charges on the SARS-CoV-2 spike. Above, "IG" in DC-SIGN" means "integrin-grabbing."

Glomerular Basement Membrane-Derived Perlecan Inhibits Mesangial Cell Adhesion to Fibronectin

Glomerular basement membrane-derived perlecan inhibits mesangial cell adhesion to fibronectin - PubMed

To explore whether the heparan sulfate proteoglycan, periecan, may act as a regulator of glomerular cell behavior, we examined the effects of perlecan on adhesive properties of rat and human mesangial cells in culture. We observed that mesangial cells did not adhere to purified perlecan...

pubmed.ncbi.nlm.nih.gov

'....The resulting avidity level is too low for mediating cell binding but sufficient to induce integrin organization into focal contacts.'

As we shall see, for the SL (SARS-like) bat virus, ZC45, there is now established a gene linking perlecan which will be shown to mimic the palsy of Parkinson's.
(TBC)

 

[badger2]

Parkinson's (PD) links to the perlecan gene of Schwartz-Jampel syndrome.

Schwartz-Jampel Syndrome (SJS)

Schwartz Jampel Syndrome - NORD (National Organization for Rare Disorders)

www.rarediseases.org

'....The classical form of SJS may be caused by changes (mutations) of a gene encoding perlecan located on the short arm of chromosome 1 (1p36.1-1-p34).'

The cell binding aspect of the RGD motif comes into view with SJS:

Baylor University / RGD Motif / Nematode Perlecan

The RGD (Arg-Gly-Asp) is a potential cell-binding motif of UNC-52/PERLECAN - PubMed

UNC-52/perlecan is a basement membrane (BM) proteoglycan playing an essential role in the muscle cell attachment of C. elegans. The UNC-52 protein contains two RGD (Arg-Gly-Asp) motifs in domains III and IV, a well-characterized tripeptide known for binding to mammalian β integrin. To...

pubmed.ncbi.nlm.nih.gov

We have already pointed to valine-to-alanine mutations in Alzheimer's and the reverse (V-to A) in this thread for juvenile AD onset as early as 26 years of age.

PINK1 for Parkinson's is located on chromosome 1p 36, linking SJS:

Juvenile Onset PD / Pink1

Juvenile-onset Parkinsonism as a result of the first mutation in the adenosine triphosphate orientation domain of PINK1 - PubMed

This study extends the phenotypic and molecular spectrum of the PINK1 gene and the geographic origin of patients with PINK1 gene mutations.

pubmed.ncbi.nlm.nih.gov

 

[badger2]

Emily Kopp's (already mentioned in this thread) and Karolina's latest work appears in this preprint:

Preprint: COVID-19 shows ‘fingerprint’ of laboratory engineering - U.S. Right to Know

COVID-19’s genome shows signs consistent with genetic manipulation, according to a new preprint.

www.usrtk.org

'....methods that keep the restriction sites were popular at the Wuhan Institute of Virology and a partnering lab in the U.S. at the University of North Carolina.'

 

[badger2]

ZC45 bat coronavirus already mentioned in this thread, is neurotropic in rats. Therefore, we will point to another RGD motif in Rattus norvegicus PINK1, because it contains a similar sequence as ZC45 spike protein (RGDP): R. norvegicus PINK1: RRSRDGP. Note that simply changing S to A yields a furin cleavage site sequence of SARS-CoV-2: (RRAR). This fact, by most anyone's estimation, would prompt lab studies of this bat coronavirus ZC45 from Zhoushan Island, Zhejiang Province.

 

[badger2]

As far as is known, neither Ebright, Quay, Kopp, Bruttel or others have analyzed the RGD sequence implications of Zhoushan bat coronavirus ZC45:
@12h

https://twitter.com/VBruttel

'....They had two brilliant ideas:
- discussing a bioengineering paper with bioengineers
- checking if exactly such RBD/FCS screening experiments were planned by virologists.'

 

[badger2]

Washburne is retweeting Bruttel:

@12h

https://twitter.com/WashburneAlex