Snake Meat......source of chinese virus

[badger2]

We have sent a communication to Italy in regard to the subspecies and type locality of Bungarus multicinctus wanghaotingi. It may not have been investigated.

11 Feb 2020 Coronavirus Likely Jumped from Bats to an 'Intermediate Host' Before Infecting Humans, WHO Says
Coronavirus likely jumped from bats to an 'intermediate host' before infecting humans, WHO says
'....Scientists are running tests on various animals, but have so far not found the host responsible for the outbreak.'

 

[badger2]

Germany asks the question about China changing the reporting numbers @ timpoint 26:33 in the video:

6 hrs ago WHO Briefing

 

[badger2]

Looking back at post #7 in this thread, is the cross-species study of Ji, et al for Bungarus multicinctus, one of the co-authors is from Nanning, as we noted. Nanning is halfway between the type location for Bungarus in Yunnan and Shenzhen,all three locations basically just below the Tropic of Cancer in latitude. At Avian Flu Talk, we retrieved Twitter footage from inside China (caution: graphic images) in the thread Breaking News Twitter Sources, the first post and the URL for "mobile twitter IsChinar" Harry Chen PhD shows feeds (scroll down near the bottom) on 8th and 7th Feb, especially see "8 Feb Listen" apparently thousands of quarantined people screaming from their enclosures. On 7 Feb, "WHO and Chinese officials reports reported 0 cases in Shenzhen, but I know 3 personal friends that have confirmed cases in all their condos....something is fishy in the shadow of Shenzhen Bay. It's very much in Shenzhen too."

As far as we can tell from the report in post #377, there are two bat species involved: '....Two specific specimens gathered in 2013 and 2015 in China from the bat species Rhinolophus affinis and R. sinicus appear to be located between the Bat coronavirus and the human 2019-nCoV groups, supporting the notion of zoonotic transfer from bats to humans (4).....and EPI_ISL_406592(collected in Shenzhen, China).'

We see that 'bat/Yunnan/RaTG13/2013/EPI_ISL_402131' from the same report may thus be Rhinolophus affinis, tentatively linking R. affinis with Bungarus multicinctus wanghaotingi in Yunnan.

 

[badger2]

Chen (IsChinar, post #383) states on 8 Feb "The Spread North. Northern China isn't prepared. The virus hits the streets in markets across the north of China, the virus is said to survive longer on cold surfaces."

We mentioned papain-like1 in post #284, and papaya potyvirus in post #261. We'll add this report to the Bungarus file:

Jul 2019 Bungarus caeruleus Venom Neutralization Activity of Azima tetracantha Lam. Extract
Bungarus caeruleus venom neutralization activity of Azima tetracantha Lam. Extract. - PubMed - NCBI

Pubmed search 'azima virus,' yields one reference, though we are yet to identify the virus that is neutralized:
Antiviral Chemotherapy V. Further Report on Flavonoids
Antiviral chemotherapy. V. Further report on flavonoids. - PubMed - NCBI

Azima tetracantha (Salvadoraceae) (syn. Monetia barlerioides L'Her) "Bee Sting Bush"

Phytochemistry V. 18: 651 New Macrocyclic, Delta-1 Piperideine Alkaloids from Papaya Leaves
'....Dehdrocarpaine I & II....other naturally occurring piperidine alkaloids with similar structures to that of carpaine have been reported in Azima tetracantha Lam. (Salvadoraceae) Rall G.J.H., Tetrahedron Letters (1965): 3465.'

Papaya Ringspot Virus
Papaya ringspot virus - Wikipedia

 

[badger2]

Post #115 of the Official Coronavirus thread, the same poster again states that Gates owns the patent on the virus, which is incorrect, as we have shown in this thread. The Pirbright Institute was studying infectious bronchitis virus (IBV) in poultry.

 

[badger2]

For those who are doing research, the Shenzhen virus is named 'Shenzhen/SZTH-001/2020/EPI_ISL_406592' while the Australian strain is named 'Australia/VIC01/2020/EPI_ISL_406844.'

 

[badger2]

We'll see if there are any mutational clues available from Los Alamos.

12 Feb 2020 Promedmail: HPAI H5N6 Avian Influenza, Qingzaigong Village, Fenming Township, Xichong, Nanchong, Sichuan
....
"Why are there 2 names for the coronavirus epidemic: "SARS-CoV2" and "CoVID-19"?

AvianFluTalk: Los Alamos National Lab, The Novel Coronavirus, 2019-nCoV, is HIghly Contagious and More infectious Than Initially Estimated
The Novel Coronavirus, 2019-nCoV, is Highly Contagious and More Infectious Than Initially Estimated - Los Alamos National Labratory : ChinaFlu

 

[badger2]

www. JAMA Network: Preparation for Possible Sustained Transmission of 2019-nCoV: Lessons from Previous Epidemics

COVID2019 First Case in London

twitter.com/jenniferatntd 'The reason why CCP held on releasing info about the coronavirus outbreak is that they were waiting for Dr. Shi Zhengli's paper to be published in Nature so that they could claim bat is the origin of COVID2019....And the best coronavirus coverage in English is the

7 Feb 2020 The Mysterious Origin of the Wuhan Coronavirus: China Continues to Refuse to Release Animal Sampling Testing Data
https://epochtimes.com/the-mysterio...lease-animal-sample-testing-data_3231298.html
'....Official Chinese state news agency, Xinhua, reported 26 Jan that 33 samples out of 585 environmental samples collected at the Huanan Seafood market were positive for nucleic acids from the new coronavirus, suggesting that the virus originated from wild animals or stocks sold there. However, these samples were from the environment, not from animals....Dr. Guan Yi pointed out that with the market now closed, it would be difficult to investigate the origin of the virus.
....
The Huge Risks of Not Identifying the Original or Intermediate Host Animals.

Given the fact that coronavirus was detected from the feces of patients from Shenzhen and that bat SARS-like virus strains were isolated from bat feces, the possible fecal-oral route of 2019-nCoV transmission in addition to respiratory droplet transmission would lead to a reasonable warning for people to avoid contact with animals like rats....Second, no bats were sold or found at the Huanan Seafood market....'

 

[badger2]

BBQed What?

10 Feb 2020 9 Family Members in Hong Kong Infected with Coronavirus After Sharing Hot Pot and BBQ
9 family members in Hong Kong infected with coronavirus after sharing hot pot & BBQ

 

[badger2]

Stolen from Avian Flu Talk to expand (and thus, condense) the investigation:

Harry Chen PhD (@IsChinar) | Twitter

ɪᴀɴ ᴍ ᴍᴀᴄᴋᴀʏ, ᴘʜᴅ (@MackayIM) | Twitter

Helen Branswell (@HelenBranswell) | Twitter

Anne Huang| 黃安安 (@drannehuang) | Twitter

Ashley Price (@davidashprice) | Twitter

thelonevirologist (@thelonevirologi) | Twitter

Catherine Moore (@SmallRedOne) | Twitter

Alison Peel (@ali_bat) | Twitter

Emanuele Capobianco (@ecapobianco) | Twitter

Kenneth Baillie (@kennethbaillie) | Twitter

James Chau (@jameschau) | Twitter

John Nkengasong (@JNkengasong) | Twitter

Prof Didier Pittet (@DidierPittet) | Twitter

https://twitter.com/FluTrackers

https://twitter.com/billcarlosbills

https://twitter.com/aetiology

 

[badger2]

We first noted 9 day survival on inanimate surfaces in post #324, though we were tracking papain mechanisms ( post #316) which was the papain mechanisms trajectory. This leads to highest thusfar for alphacoronavirus 1, TGEV, mentioned in posts #343 & #344, and MHV (mouse hepatitis coronavirus,[cold-shock trajectory])
for this German study:

6 Feb 2020 Germany
Persistence of coronaviruses on inanimate surfaces and its inactivation with biocidal agents. - PubMed - NCBI
'....Table 1. On steel: TGEV 4 C, 20C, 28 days; MHV 4C, 20C 28 days....HCoV 229E On plastic 5-9 days room temperature,on silicon 21C 5 days, on pvc 21C 5 days.'

 

[badger2]

If readers had read post #324, they would already know that it referred to the 6 Feb 2020 study from Germany: '....to dispel all the fake news and unreliable information online even from so-called viral expert organizations, a team of researchers from Greifswald and Bochum, Germany....' The rest of the post #324 article shows that hidden inside the coronavirus genome are mechanisms for 28-day survival on surfaces.

 

[badger2]

The report in the USMB Official Coronavirus thread, post #121, states....'median incubation period was 3.0 days (range 0 to 24.0).'

 

[badger2]

1,000 LA People to Self Quarantine
Twitter

 

[badger2]

Remember, the 24-day incubation of the Official Coronavirus thread is a cut-off point, and we are not told how many patients were near that extreme, which biases the median of about 3 days. And in another thread, no, comparing influenza to coronoavirus is not stupid. We have already posted the study by Ji et al for codon usage by one of the suspected intermediate hosts of 2019-nCoV (COVID-19), Bungarus multicinctus.

In addition, vaccine manufacture is assisted by codon usage studies such as....

Genome-Wide Analysis of Codon Usage Bias in Ebolavirus, Virus Research (2015) 196: 87-93

Synonymous and Biased Codon Usage by MERS CoV Papain-Like and 3CL-proteases, Biol. Pharm. Bull. (Japan) (2017) 40: 1086-91

Genomic Analysis of Codon Usage Shows Influence of Mutation Pressure, Natural Selection, and Host Features on Marburg Virus Evolution, BMC Evol. Biol. (2015) 15: 174

Codon Usage Bias and the Evolution of Influenza A Viruses. BMC Evol. Biol. (2010) 10: 253

 

[badger2]

13 Feb 2020 15th Case, San Antonio, Tx.

An atomic difference trajectory is at posts # 245 (bat influenza) and #205 (bungarotoxin/influenza A).

 

[QuickHitCurepon]

This thread ever gonna die? Sorry, just sik of seeing it

badger doesn't think so. lol

 

[badger2]

Serious readers are suggested to visit Avian Flu Talk, and the thread, "Hubei Adds 15,000 New Cases." In that thread is a graph of ethnicity. We have already mentioned ACE2 in this thread, though surprisingly, Asians have more ACE2 receptors than others, with important ethnic differences, in this order:

Japan, Tokyo

Southern Han

East Asian
Kinh in Ho Chi Minh City, Vietnam

Chinese Dai in Xishuangbanna
(1000 Genomes Project)

We are given rs numbers in the graph. Noting that the second rs number links to the area in Yunnan for the type locality of Bungarus multicinctus wanghaotingi: Yuankiang (other spelling, Yuanjiang?), which could mean the entire stretch of the Yuan Jiang in Yunnan. The bat virus closest to humans (previous post) has not been identified as yet to locality except that is was apparently collected in Yunnan in 2013. The center of the Xishuangbanna area is along the Lancang Jiang (river). Yuanjiang municipality is northeast of the Xishuangbanna are, less tyhan 60 miles. The Yuan Jiang (River) begins at Midu and runs into the Gulf of Tonkin at Hanoi, a total distance of about 500 miles. Yuanjiang is about 30 miles from this river, just over the ridge (see map), and thus about 80 miles from the Kunming Bird and Flower market, thence 500 miles to Wuhan.

If the rs numbers on the graph are in order, the second-most abundant ACE2 receptor population is the Dai in Xishuangbanna, rs714205.

Nov 2017 / rs714205 / Kunming / Wa Chinese Population
Interactions between angiotensin-converting enzyme-2 polymorphisms and high salt intake increase the risk of hypertension in the Chinese Wa populat... - PubMed - NCBI

 

[badger2]

SNPs mentioned in the angiotensin study (post # 398) indeed link to codon usage bias, and the hotspots for bias studies are the Wa population and nearby snakes such as Bungarus multicintus wanghaotingi.

 

[badger2]

It doesn't always pay just to think about going back to school.

Pubmed search 'codon usage bias snps' yields 72 references, including this one:

Dec 2019 White Spot Syndrome Virus in Shrimp
Synonymous SNPs of viral genes facilitate virus to escape host antiviral RNAi immunity. - PubMed - NCBI

We have already mentioned the Penaeus monodon virus in this thread.