Snake Meat......source of chinese virus

[QuickHitCurepon]

The Chinese eat badgers.

Snakes Are The Likely Source of China's Deadly Coronavirus. Here's Why

In the case of this 2019 coronavirus outbreak, reports state that most of the first group of patients hospitalized were workers or customers at a local seafood wholesale market which also sold processed meats and live consumable animals including poultry, donkeys, sheep, pigs, camels, foxes, badgers, bamboo rats, hedgehogs and reptiles.

 

[QuickHitCurepon]

The Coronavirus is going to spare a lot of happy animals the torture of being held and killed for meat because of the ban.

China says it will ban the trade in wild animals, like bats, believed to be behind the Wuhan coronavirus, and tighten supervision on 'wet markets'

 

[badger2]

Human tests for the Inovio vaccine will begin this summer, though the reservoir remains in nature just as it does for Ebola-Marburg. Epidemiology will remain alive despite vaccines, as we have seen for the common cold and influenza. Many kinds of animals will continue to be used as food, especially in the rural parts of China. Inovio is using MERS as a model, and most likely the N protein will be paramount, because the S protein is too unwieldy:

'The N protein is the most conserved and stable protein among the CoV structural proteins; whereas the S protein undergoes several drastic changes during virus infection. Additionally, the S protein is prone to mutations, especially the amino acids associated with spike protein- cell receptor interface, in order to overcome host immunity. In an interesting study, the N gene of CoV was found to be more effective for evaluating the codon usage bias than the S gene.'
(Jan 2020, Sheikh A, et al, [Saudi Arabia], Analysis of Preferred Codon Usage in the Coronavirus N Genes and Their Implications for Genome Evolution and Vaccine Design)
Analysis of preferred codon usage in the coronavirus N genes and their implications for genome evolution and vaccine design. - PubMed - NCBI

Using codon usage, the above study from Saudi Arabia will link to the serine-to-leucine of Orf 8 of 2019-nCoV (COVID-19 ).

11 Feb 2020 The Official Name for the Disease
Overview of The 2019 Novel Coronavirus (2019-nCoV): The Pathogen of Severe Specific Contagious Pneumonia (SSCP). - PubMed - NCBI
'....Severe Specific Contagious Pneumonia (SSCP).'

 

[badger2]

The snake report in post #401 is way late. We posted that report (Ji, et al) in post #7. We find a Kunming link, which is the location of the notorious Bird and Animal market (previous posts):

ACE2 / SARS-Like Coronavirus
Isolation and characterization of a bat SARS-like coronavirus that uses the ACE2 receptor. - PubMed - NCBI
'....We conducted a 12-month longitudinal survey (Ap 2011- Sept 2012) if SL-CoVs in a colony of Rhinolophus sinicus at a single location in Kunming, Yunnan Province, China....27 of 117 samples (23%) were PCR-positive and subsequently confirmed by sequencing.'

 

[badger2]

At timepoint 1:25, no species are mentioned.


In this thread, we have already found the exact location in Yunnan of a second subspecies of Chinese krait (Bungarus multicinctus wanghaotingi) which type locality is about 60 miles from the Xishaungbanna and Wa Chinese population. It has yet to be confirmed whether this subspecies genome has even been sequenced.

 

[badger2]

Pubmed Genome list for Bungarus yields no results.

 

[badger2]

pubmed search 'wanghaotingi' yields two references, one of which we have already excerpted in this thread:

Erythrocyte glycolipids in Huntington's chorea. - PubMed - NCBI

Estrogen stimulation of synthesis of specific proteins and RNA polymerase activity in the immature chick oviduct. - PubMed - NCBI

 

[badger2]

These are the PMID numbers (rather than PMC numbers, post #407):

Cytochrome C oxidase subunit I barcodes provide an efficient tool for Jinqian Baihua She (Bungarus parvus) authentication. - PubMed - NCBI

General Component Analysis (GCA): A new approach to identify Chinese corporate bond market structures. - PubMed - NCBI

 

[badger2]

Snakebite incidence in two townships in Mandalay Division, Myanmar. - PubMed - NCBI

 

[badger2]

One can read that they make a medicine for arthritis, rheumatism, etc. by immersing the snake in strong alcoholic drinks for months.

 

[badger2]

Wikipage mentions the subspecies, though as we have seen in other posts, there are morphological differences distinguishing the two: B.b. wanghaotingi has absence of loreals and differing morphology of the preocular area.

Many-Banded Krait
Many-banded krait - Wikipedia

 

[Wapasha]

'Interestingly, no similar sequence was found with known sequence in the database, suggesting that a putative recombinant parent virus was still unknown.'

Avian Flu Talk: 'The Independent understands that public health officials are making attempts to trace as many as 2,000 people who have arrived in the UK from Wuhan.'

12 Possible U.S. Cases

The Chinese people are the source, they are nothing but a manufacturing tool and breeding ground for these viruses.

 

[badger2]

In post #313, the serine-to-leucine in Orf8 of COVID-19 was mentioned, and ACE2 was coupled to leucine in post #314. From the Saudi study on codon bias, indeed, both are mentioned first:

Analysis of preferred codon usage in the coronavirus N genes and their implications for genome evolution and vaccine design. - PubMed - NCBI
'....Results....The amino acid Leu overbiased with CUU codon in all genes except in hCov HKU1 where it overbiased with UUA. The amino acid Ser overrepresented with UCU in all except in ibCoV (infectious bronchitis virus) and bCoV (bat coronavirus).'

The 'HK' is misleading: hCoV HKU1 came from Shenzhen:

Human coronavirus HKU1 - Wikipedia
'....2005....the man had recently returned to Hong Kong from Shenzhen. This is in line with the latitudes we have already mentioned for Nanning and Yuanjiang.

 

[badger2]

Current stats (post #279): 60,416 cases, 1,370 deaths.

AFT: 'Twitter sources: Hundreds of fires breaking out over China, lines of people for water, people hunted down and beaten for violating quarantine, thousands and thousands of migrant workers sleeping outside, thousands of communist party banners strung everywhere depicting any resistance as class enemies and threatening violence, death to whole villages if anyone escapes, bounties for anyone reporting a fever, thousands of "volunteers" sent to Wuhan, military airlifts, police official collapses in Xinjiang (home to a million Uighurs in concentration camps), people lining up to buy eggs at $1 each....'

 

[badger2]

Bungarus multicinctus wanghaotingi is not mentioned in GenBank database, so the two different genomes are not comparable at this time. There are supposedly morphological differences, so there should be genomic differences.

Because a coronavirus (TGEV) can survive on surfaces for up to 28 days, we retrieve the cold-shock trail linking hepatitis B virus to the mulberry tree (posts #11, 112, 133, 139), while moving the investigation from Current Events to the Music forum because there are sequences to select and play. One of these sequences is from Bungarus fasciatus C-type lectin which links to ebola and banana lectin, human lectins, etc. and we have already mentioned frugiperda moth cells involved in protein studies for COVID-19 (formerly 2019-nCoV) in this thread.

The cold-shock trail involves mulberry and the moth that feeds on the tree.

'In Bombyx mori, the ENC values varied from 51-60 with an average of 29.47 (Wei, et al, 2014)....Complete nucleotide coding sequences of genes from four selected Bungarus species namely B. multicinctus, B. fasciatus, B. candidus, and B. flaviceps were retrieved from National Center for Biotechnology Information (NCBI)
(https://www.ncbi.nlm.nih.gov)GenBank database.
....
Low codon usage bias might be useful for efficient replication in Bungarus species with different cell types having different choices of codon usage. Further, low codon bias indicates the existence of almost all synonymous codons for an amino acid and it reflects the presence of greater genetic variability of the coding sequence in terms of codon usage. High genetic variability widens the platform for evolution to occur primarily by mutation pressure and natural selection.'
(Chakraborty S, et al, Codon Usage Pattern and Prediction of Gene Expression Level in Bungarus Species)
Codon usage pattern and prediction of gene expression level in Bungarus species. - PubMed - NCBI

 

[badger2]

COVID-19 vector/reservoir in nature must be found due to the possibility of vaccine revertants.

14 Feb 2020 Reinfection Deadlier
https://www.taiwannews.com/tw/news/3876197
'...."outsmarted all of us" as it can hide symptoms for up to 24 days.'

And perhaps survive up to 28 days (post #391)

 

[badger2]

URL was correctly transcribed. www. for "Exclusive: Chinese Doctors Say Wuhan Coronavirus Reinfection Even Deadlier"

 

[badger2]

Apparently at Pubmed, there are no references to ebola revertants, though there are references to coronavirus revertants:

Avian Flu Talk, thread "More on COVID-19" '....We still have no evidence that this virus affects domestic animals, but since we also still have no real evidence that it doesn't. it's best to continue to make plans to reduce risk of exposure....On the prevention front, my dog Merlin had fake coronavirus for the day (just like he had fake ebola a few years ago) to practice our containment practices. He escaped unscathed (and managed to make the front page of the Toronto Star).'

Singapore: Grace Assembly Church and DBS Bank
Coronavirus: 8 new cases in Singapore, including 5 more linked to Grace Assembly church, 1 linked to DBS case
'....another two cases are Bangladeshi work-pass holders....'

Oct 2015 Spain/Iowa SARS Vaccine Revertant
Identification of the Mechanisms Causing Reversion to Virulence in an Attenuated SARS-CoV for the Design of a Genetically Stable Vaccine. - PubMed - NCBI
'....This mutant virus regained fitness....SARS-CoV-delta E protein 8a mutated, to now encode a PBM (PDZ-binding motif), and also regained virulence.'

Jul 2018 India / Infectious Bronchitis Coronavirus
Emergence of a genotype I variant of avian infectious bronchitis virus from Northern part of India. - PubMed - NCBI
'....the deduced amino acid sequence of S gene of IBV isolates showed a high level of identity with strains from Tamil Nadu and the reference vaccine strains....A revertant strain originally evolved from the live attenuated vaccine strains.'

 

[badger2]

This URL will function if typed in the spacebar:
gzscbm.com/web/technique/792.html
'....Researchers from Huazhong Agricultural University analyzed and compared the entire genome sequence of the GISAID database 2019-nCoV and found that it is highly similar to the known Yunnan bat Beta-Coronavirus (BetaCoV/bat/yunnan/ratg13/2013/epi_isl_402131) reaching 96% - 96.1%.....On 7 Feb, South China Agricultural University and other units conducted metagenomic analysis of wild animal samples and identified the genetic sequence of the coronavirus strain isolated from pangolins as high as 99% with 2019-nCoV, indicating that pangolins are the potential intermediate host..'

The study that mentioned coronavirus infection in pangolins (previous post) did not identify what types of coronaviruses in their report.

Huazhong Agricultural University
Huazhong Agricultural University - Wikipedia

On the Origins of 2019-nCoV
On the Origins of the 2019-nCoV Virus, Wuhan, China | PSI Intl
'....This led to criticism on Wired (3) with quote dismissing the snake origins hypothesis as lacking evidence....2019-nCoV could become the worst example of vaccine-derived contagious disease in human history.'

The principia-scientific report mentions a lab-derived insertion.

 

[badger2]

Badger2 supports Gao's decision. More on the snake hypothesis:
No, the Wuhan Virus Is Not a 'Snake Flu'
'...."There's about as much justification for snakes being the intermediate hosts as me saying on a whim right now that it's birds," he says....They believe the speed-up was appropriate. "With this situation, with people dying, holding this paper up in review would be criminal," says Shou-Jian Gao, the journal's editor-in-chief. "This is intended to just open the scientific dialogue." He invited any researchers with dissenting assessments to submit them to the journal as written commentaries.'