Snake Meat......source of chinese virus

[badger2]

Oct 2014 Bungarus parvus
Cytochrome C oxidase subunit I barcodes provide an efficient tool for Jinqian Baihua She (Bungarus parvus) authentication. - PubMed - NCBI

Sea Krait, Laticauda
Sea krait - Wikipedia
'....sea kraits are also often confused with land kraits....'

 

[badger2]

Pubmed has 5 refs for Bungarus parvus:
bungarus parvus - PubMed - NCBI

Wikipedia does not list Bungarus parvus
Bungarus - Wikipedia

 

[badger2]

Bungarus parvus
https://www.zhzyw.com/zycs/zytp/zy/0982616D94777J8BFJ2AJDA3.html

 

[badger2]

Click on the prompt for a machine translation.

 

[badger2]

We think that we have found the closest link thusfar between bat, snake and 2019-nCoV. To speed the investigation, we will mention the current suspect first along with the geography. The suspect is Bungarus multicinctus wanghaotingi. B. m. w. is a subspecies of B. multicinctus. We make 2019-nCoV distinction for geography:

(University of Bologna, Italy), Ceraolo C and Giorgi FM, Genomic Variance of the 2019-nCoV Coronavirus
'....Similar to a previous report with 10 virus specimens, we detected a very high conservation between the 56 analyzed 2019-nCoV genomes, with sequence identity above 99%. We found a bat CoV genome (Global Initiative on Sharing All Influenza Data [GSAID] EPI_ISL_402131) with 96.2% sequence identity (and query coverage above 99%) to the 2019-nCoV reference sequence (NC_045512.2), while the previously reported closest bat CoV (bat-SL-CoVZC45) has a sequence similarity of 88%. The reference human SARS genome (NC_004718.3) appears more distant from the reference 2019-nCoV, with a sequence identity of 80.26% and query coverage of 98%....While 2019-nCoV constitute a tightly similar cluster, the two bat virus sequences MG772934.1 and EPI_ISL402131 appear to be linking the human 2019-nCoV to the bat coronaviridae.'

 

[badger2]

The Figure 1 in the report of post#365 (first reported in post #313) shows relationships between strains of human 2019-nCoV and bat coronaviruses: 'bat/Yunnan/RaTG13/2013/EPI_ISL402131.'

The target geography is Yunnan Province.

Bungarus multicinctus / B. multicinctus wanghaotingi
Cytochrome C oxidase subunit I barcodes provide an efficient tool for Jinqian Baihua She (Bungarus parvus) authentication. - PubMed - NCBI
'....In China, two subspecies of Bungarus multicinctus were recorded, B. multicinctus multicinctus and B. wanghaotingi. The latter distributes only in Yunnan Province, far from the sample collection sites in Guangdong. We checked all the specimens of B. multicinctus thoroughly, and found no morphological characteristics of subspecies B. multicinctus wanghaotingi. We therefore presume that the intraspecific differentiation may be attributed to some environmental factors, especially the residential altitude of the different snake populations. The same phenomenon was also observed in other snake species (Gou P, Malhotra A).'

Three different Malhotra authors contribute to krait science:

Rational truncation of aptamer for cross-species application to detect krait envenomation. - PubMed - NCBI

Non-invasive ventilation for acute respiratory failure due to a snakebite. - PubMed - NCBI

Neuromuscular transmission failure due to common krait (Bungarus caeruleus) envenomation. - PubMed - NCBI

From the California Academy of Sciences (San Francisco) and the National Museum of Natural History (Washington, D.C.), this report gives further information on the identification and geography of the suspect snake:

'Bungarus multicinctus wanghaotingi Pope 1928 Type Locality: Yuankiang, Yunnan, China; Holotype: AMNH35230
....
B. m. w. 27-48 narrow white crossbars on back, crossbars narrowest mid-dorsally, expanding on sides....Loreal absent, nasal in contact with preocular; vertebral scales distinctly enlarged. (Key to the Genera and Species of Venomous Snakes of Myanmar)
(Leviton AE, et al, The Dangerously Venomous Snakes of Myanmar: Illustrated Checklist with Keys, PCAS Vol 54 [2003] : 407-462)

 

[badger2]

While we have no access to the geography of (2013) EPI_ISL 402131 that came from somewhere in Yunnan, there is more info on Yuankiang, which supports a change in temperature (cold-shock, altitude, temperature-sensitive mutants, etc.):

Yuanjiang, Yunnan
https://en.wikipedia.org/wiki/Yuangjiang_Hani,_Yi_and_Dai_Autonomous_County
'....lies at the junction of three different climate types....deep mountainous river valley....'

 

[badger2]

The webpage with map can be retrieved here:

List of Administrative Divisions of Yunnan
List of administrative divisions of Yunnan - Wikipedia
'Yuxi City'

 

[badger2]

Firstly, distance from Kunming to Wuhan is 802 miles.

 

[badger2]

Awful Animal Market --Review of Flowers and Birds Market of Kunming
Awful animal market - Review of Flowers and Birds Market of Kunming, Kunming, China - Tripadvisor
' 71 reviews:....often filthy with stall holders showing little concern for their welfare....I saw snakes and many (what I assume are) wild-caught birds for sale along with orchids which were clearly ripped from trees in the jungle.'

 

[badger2]

From Avian Flu Talk, it seems that Plummer was in Nairobi for a conference:

6 Feb 2020 Frank Plummer Dead
https://www.greatgameindia.com/fran...ey-to-coronavirus-investigation-assassinated/

 

[badger2]

Frank Plummer - Canadian Scientist Key to Coronavirus Investigation Assassinated in Africa?
Frank Plummer - Canadian Scientist Key To Coronavirus Investigation Assassinated In Africa? | GreatGameIndia

 

[badger2]

Now that we have the URL up and working, the reader is invited to click on 'Indian scientists found HIV-like injections in Wuhan coronavirus,' which will link to post #365 from Bologna. We can clearly see the virus in question in that report, which reads....'bat/Yunnan/RaTG13/2013/EPI_ISL_402131.'

Next, this report, if the URL functions:

Uncanny Similarity of Unique Inserts in the 21019-nCoV Spike Protein to HIV-1 gp120 and Gag
https://www.biorxiv.org/content/10.1101/2020.01.30.92787v1#comment-4779677688

 

[badger2]

Avian Flu Talk: "It appears to be an Indian conspiracy theory site, so I would say no, it's not credible."

We will try to unravel the recent trajectory we took from the GreatGameIndia webpage (post #372). We will end up at Twitter and the comments there, which will link back precisely to post #365 of this thread.

 

[badger2]

For those who will investigate the trajectory, the comments at various places for the report in post #373 will retrieve things such as this:

'2019-n-CoV isoforms: GTNGTKR, HKNNKS, GDSSSG, QTNSPRRA.' These are the apparent insertions ('injections') for the bioweapons theory. However, what we found were Twitter entries such as....'Joining Unrelated Dots "ngschen a day ago: " This paper is fake. I aligned all 4 insertions, 3 of them share with bat SARS-like virus (GSAID no.: BetaCoV bat Yunnan RaTG13/2013 EPI_ISL_402131. 4th insertions which is the only one 2019-nCoV specific insertion, has the best match to marine virus AFVG_250M1136. Since the 2019-nCoV outbreaks from the seafood market, the possibility of marine virus recombination might be much more persuasive.'
Twitter.com/Mareeswj/status/1223854757283954689/photo/1

The full entry for the report in post #365 is 'bat/Yunnan/RaTG13/2013/EPI_ISL_402131.' This is the target virus we have built today's investigation upon. As far as the boundaries of China go, Bungarus multicinctus wanghaotingi Pope, 1928 only occurs in Yunnan Province.

 

[badger2]

WHO must have seen some scarey things when it went to China. This from AFT:

11 Feb 2020 WHO Warns of 'Very Grave' Global Virus Threat
WHO warns of 'very grave' global virus threat

 

[badger2]

If the type locality for Bungarus multicinctus wanghaotingi is Yuankiang, Yunnan Province, China, the closest spelling is Yuanjiang, near the Yuan River, Yunnan (spelled Yuan Jiang['river] which flows into Hanoi, North Vietnam). The closest point in Yunnan to Wuhan is about 500 miles, recalling that 2019-nCoV can remain viable on surfaces for 9 days. Thus, the range of the subspecies Bungarus multicinctus wanghaotingi comes into question.

We have already mentioned pharmacies as potential reservoirs due to TCM.

10 Feb 2020 China Stops Medicine Sales
Chinese cities try to flush out coronavirus patients by stopping cough medicine sales
'....Southern Guangdong Province, home to the tech hub Shenzhen that borders Hong Kong, has asked residents to register with their real names at medicine stores when they purchase so officials can follow up with them.'

However, the Chinese may has suspicions, because Guangdong Province has already been mentioned in this thread as collection points for snakes, and the Italians have the Shenzhen strain:

'The catPCA analysis on the sole 2019-nCoV sequences highlights some internal variability (Figure 2B), with two likely outliers identified in the genome EPI_ISL_406862 (collected in Germany) and EPI_ISL_406592 (collected in Shenzhen, China).'
(Ceraolo and Giorgi, Genomic Variance of the 2019-nCoV Coronavirus, op cit)

 

[badger2]

Here is a snippet from AFT: "This thing hit mainstream 21 days ago....it feels like 9 months. Go back and do a snapshot of world events, and this 21 days ago....surreal."

11 Feb 2020 China Changes Counting Scheme for Wuhan Virus
https://taiwannews.com.tw/en/news/3874490

 

[badger2]

China changes counting scheme to lower Wuhan ... | Taiwan News

 

[badger2]

This expert hasn't explained the reservoir in Nature, nor the temperatures involved when 2019-nCoV could be hiding under snake or pangolin scales, either in a market or in the wild:

Coronavirus Expert Says He Knows When the Virus Will 'Burn Itself Out'
Coronavirus expert says he knows when the virus 'will burn itself out,' according to leaked analysis
'....The virus can remain intact at 4 degrees (39 F) or 10 degrees (50 F) for a longer period of time. But at 30 degrees (86 F) you get inactivation.'