Doctors are coming out in droves saying hydroxychloroquine works

[badger2]

For example, the article in post #1,522 is about glycosylation, which is a major prognostic factor. HCQ does change endosome pH, though the virus can switch from ACE2 receptors to TMPRSS2, and TMPRSS2 connects to a current suspect as intermediate host of COVID-19, the Yangtze finless porpoise. All of this links to the Wuhan-Pennsylvania connection, already mentioned on the snake meat thread:

Bat SARS-Like WIV1 coronavirus uses the ACE2 of multiple animal species as receptor and evades IFITM3 restriction via TMPRSS2 activation of membrane fusion - PubMed

Diverse SARS-like coronaviruses (SL-CoVs) have been identified from bats and other animal species. Like SARS-CoV, some bat SL-CoVs, such as WIV1, also use angiotensin converting enzyme 2 (ACE2) from human and bat as entry receptor. However, whether these viruses can also use the ACE2 of other...

www.ncbi.nlm.nih.gov

Furthermore, it is dumb not to include zinc and azithromycin in arguing for the benefits of hcq: the Zelenko protocol.

 

[ABikerSailor]

Most doctors and scientists are saying that HCLQ doesn't provide any significant benefit. If I catch this thing, I hope they use Remdosivir or plasma transfusions to treat me. HCLQ is bullshit and doesn't really work.

 

[badger2]

It's obvious that post # 1,542 has not read Gold's white paper on hydroxychloroquine. The way forward is to test individual posters so that other readers can actually see who is clued up and who is not. Remdesivir and dexamethasone discourse should also be welcome, though the jury is still out on other treatments, such as the new Russian areplivir (based on favipiravir).

It's interesting that, even after Homo sapiens has learned the history of cocktails against HIV-1, it's still having trouble accepting complexities. It wants a once-and-for-all answer for itself.

 

[ABikerSailor]

It's obvious that post # 1,542 has not read Gold's white paper on hydroxychloroquine. The way forward is to test individual posters so that other readers can actually see who is clued up and who is not. Remdesivir and dexamethasone discourse should also be welcome, though the jury is still out on other treatments, such as the new Russian areplivir (based on favipiravir).

It's interesting that, even after Homo sapiens has learned the history of cocktails against HIV-1, it's still having trouble accepting complexities. It wants a once-and-for-all answer for itself.

You can always find some idiot to support your bullshit. Doesn't mean you are right. Remember when some doctors said that smoking is good for you back in the 50's and early 60's?

 

[badger2]

In addition, once the source of COVID-19 in nature is discovered, treatment should advance greatly, including vaccines (COVID-19 hamster model may be possible). Is there a valid reason for repeating the embarassing history of the source of ebola in nature?

 

[badger2]

What bullshit we're dealing with is 'god is my sensei' xian bigotry, scared shitless to go back to school.

 

[JimZiegelbauer]

Trump motivated by army of doctors, triggers Left with tweet binge on hydroxychloroquine evidence

Leftists got triggered after Trump retweeted the White Coat Summit, where an army of coronavirus experts declared that hydroxychloroquine works.

www.bizpacreview.com

View attachment 368204
Democrats get
Triggered

Democrates and CNN were made fools of AGAIN

 

[ABikerSailor]

In addition, once the source of COVID-19 in nature is discovered, treatment should advance greatly, including vaccines (COVID-19 hamster model may be possible). Is there a valid reason for repeating the embarassing history of the source of ebola in nature?

How in the hell is a hamster model going to work for a virus that originated in bats?

 

[badger2]

Post #1,548: If you would have read the snake meat thread, you'd know how the hamster model fits into the drama.

 

[TroglocratsRdumb]

The Antifacrats would rather people slowly suffocate to death than admit that President Trump is correct.

 

[badger2]

Anyone contemplating plasma in lieu of hydroxychloroquine should know about the D614G mutation that occurred in COVID-19 at least by Feb 2020.

COVID-19 / Convalescent Plasma

The D614G mutation in the SARS-CoV-2 spike protein reduces S1 shedding and increases infectivity - PubMed

SARS coronavirus 2 (SARS-CoV-2) isolates encoding a D614G mutation in the viral spike (S) protein predominate over time in locales where it is found, implying that this change enhances viral transmission. We therefore compared the functional properties of the S proteins with aspartic acid (S...

www.ncbi.nlm.nih.gov

So we see the virus mutate towards alkalinity, because its original aspartic acid (D, pH 2.98, acidic) went higher, to glycine (G, pH 6.06, more basic), though hcq (also [italics]) increases alkalinity in endosomes. This hcq-induced alkalinity affects virus replication.

 

[colfax_m]

For example, the article in post #1,522 is about glycosylation, which is a major prognostic factor. HCQ does change endosome pH, though the virus can switch from ACE2 receptors to TMPRSS2, and TMPRSS2 connects to a current suspect as intermediate host of COVID-19, the Yangtze finless porpoise. All of this links to the Wuhan-Pennsylvania connection, already mentioned on the snake meat thread:

Bat SARS-Like WIV1 coronavirus uses the ACE2 of multiple animal species as receptor and evades IFITM3 restriction via TMPRSS2 activation of membrane fusion - PubMed

Diverse SARS-like coronaviruses (SL-CoVs) have been identified from bats and other animal species. Like SARS-CoV, some bat SL-CoVs, such as WIV1, also use angiotensin converting enzyme 2 (ACE2) from human and bat as entry receptor. However, whether these viruses can also use the ACE2 of other...

www.ncbi.nlm.nih.gov

Furthermore, it is dumb not to include zinc and azithromycin in arguing for the benefits of hcq: the Zelenko protocol.

I'm not sure you actually read the paper you're referencing. For starters, it's not a paper about COVID 19. Second, there is no "switching from ACE2 to TMPRSS2" since TMPRSS2 is a protease and not a binding site.

 

[badger2]

At avian flu talk forum, they are hawking green tea as a zinc ionophore, because zinc is difficult to get into the cell. However, chloroquine is also a zinc ionophore.

Zinc / Chloroquine / Ionophore

Can Zn Be a Critical Element in COVID-19 Treatment? - PubMed

The current COVID-19 pandemic caused by SARS-CoV-2 has prompted investigators worldwide to search for an effective anti-viral treatment. A number of anti-viral drugs such as ribavirin, remdesivir, lopinavir/ritonavir, antibiotics such as azithromycin and doxycycline, and anti-parasite such as...

www.ncbi.nlm.nih.gov

'....using chloroquine as an ionophore while zinc inside the infected cell can stop SARS-CoV-2 replication.'

 

[badger2]

#1,552: You are incorrect. COVID-19 can use TPRSS2 as an alternate enzyme. It does not need ACE2.

 

[badger2]

The Conundrum of the Binding Site

Can COVID-19 gain cell entry without S priming/TMPRSS2?

TMPRSS2

https://en.wikipedia.org/TMPRSS2

Ap 2020 SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2

SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor - PubMed

The recent emergence of the novel, pathogenic SARS-coronavirus 2 (SARS-CoV-2) in China and its rapid national and international spread pose a global health emergency. Cell entry of coronaviruses depends on binding of the viral spike (S) proteins to cellular receptors and on S protein priming by...

www.ncbi.nlm.nih.gov

 

[badger2]

It needs both.

 

[badger2]

The article of post #1,555 continue, recalling that ebola vaccine is based on VSV:

'....Replication-defective VSV particles bearing coronavirus S proteins faithfully reflect key aspects of coronavirus host cell entry....All cell lines were readily susceptible to entry driven by the glycoprotein of the pantropic VSV (VSV-G), as expected....In order to determine whether SARS-CoV-2 can use CatB/L for cell entry, we initially employed ammonium chloride, which elevates endosomal pH and thereby blocks CatB/L activity....only TMPRSS2 activity is essential for viral spread and pathogenesis in the infected host whereas CatB/L activity is dispensable....Priming of coronavirus S proteins by host cell proteases is essential for viral entry into cells, and encompasses S protein cleavage as the S1/2 and S2' sites. The S1/2 cleavage site of SARS-CoV-2-S harbors several arginine residues (multibasic), which indicates high cleavability. Indeed, SARS-CoV-2 was efficiently cleaved in cells and cleaved S protein was incorporated into VSV particles. Notably, the cleavage site sequence can determine the zoonotic potential of coronaviruses, and a multibasic cleavage site was not present in RaTG13, the coronavirus most closely related to SARS-CoV-2. It will be interesting to determine whether the presence of a multibasic cleavage site is required for SARS-CoV-2 entry into human cells and how this cleavage site was acquired.'

Arginine (R) has the highest isoelectric pH, and we first mentioned RaTG13 in post #366 of the snake meat thread. RaTG13 was from the Yunnan bat. This zoonotic potential of the cleavage site may help pinpoint the intermediate COVID-19 host in nature.

 

[badger2]

In another thread, we have already mentioned lungworm of suspected COVID-19 intermediate host, Neophocaena and the swine lungworm as intermediate host of influenza virus. Thus for the TMPRSS2 investigative trajectory, there is an entry for bat-associated influenza and TMPRSS2:

Hemagglutinin of Bat-Associated Influenza Virus is Activated by TMPRSS2 for pH-Dependent Entry Into Bat but not Human Cells

The Hemagglutinin of Bat-Associated Influenza Viruses Is Activated by TMPRSS2 for pH-Dependent Entry into Bat but Not Human Cells - PubMed

New World bats have recently been discovered to harbor influenza A virus (FLUAV)-related viruses, termed bat-associated influenza A-like viruses (batFLUAV). The internal proteins of batFLUAV are functional in mammalian cells. In contrast, no biological functionality could be demonstrated for the...

www.ncbi.nlm.nih.gov

 

[badger2]

We've already mentioned ebola, CatB/L and VSV. TMPRSS2 links ebola drugs such as remdesivir:

Ebola / Marburg / TMPRSS2

Cathepsins B and L activate Ebola but not Marburg virus glycoproteins for efficient entry into cell lines and macrophages independent of TMPRSS2 expression - PubMed

Ebola (EBOV) and Marburg virus (MARV) cause severe hemorrhagic fever. The host cell proteases cathepsin B and L activate the Zaire ebolavirus glycoprotein (GP) for cellular entry and constitute potential targets for antiviral intervention. However, it is unclear if different EBOV species and...

www.ncbi.nlm.nih.gov

Chagas disease links a parasite (trypanosome) in comparison to hcq for malaria:

Chagas / Ebola / CatB/L

Protease inhibitors targeting coronavirus and filovirus entry - PubMed

In order to gain entry into cells, diverse viruses, including Ebola virus, SARS-coronavirus and the emerging MERS-coronavirus, depend on activation of their envelope glycoproteins by host cell proteases. The respective enzymes are thus excellent targets for antiviral intervention. In cell...

www.ncbi.nlm.nih.gov

 

[badger2]

Nafamostat is promising and here also is mentioned remdesivir and chloroquine in comparison, remdesivir has a higher SI (selectivity index) than chloroquine, though we don't know the SI for hydroxychloroquine:

COVID-19 / Nafamostat / Remdesivir / Chloroquine

Remdesivir and chloroquine effectively inhibit the recently emerged novel coronavirus (2019-nCoV) in vitro - PubMed

Remdesivir and chloroquine effectively inhibit the recently emerged novel coronavirus (2019-nCoV) in vitro

www.ncbi.nlm.nih.gov

'....Notably, two compounds, remdesivir (EC50= 0.77 mM, SI 129.87) and chloroquine (EC50= 1.13 mM, SI 88.50) potently blocked virus infection at low micromolar concentration and showed high SI.'