COVID-19’s Biological Politics

Next linking lactate dehydrogenase to elevated levels in SARS-CoV-2-infected Italian patients:

Aug 2021 Italy / Sex Differences During First and Second Wave of the Pandemic
’....Ferritin and alanine aminotransferase (ALT) were also higher in males, but constant in the two waves, while lactate dehydrogenase (LDH) levels were similar in males and females, but higher in patients hospitalized during the first wave....The highest levels of WBC, neutrophils, CRP, fibrinogen, D-dimer, ferritin, LDH and procalcitonin were preferentially reported in patients admitted in ICU than those that were managed in the other hospital units, or who died during the course of COVID-19....more males showed interstitial pneumonia, while more females reported vomiting.’

MacLachlan, et al, above, continues:

’The initial isolates of PRRSV from North America and Europe were very different; indeed, the European viruses were almost as distinct from the North American PRRSV isolates as they were from LDV. PRRSV isolates from other regions of the world were similar to the North American genotypes. The marked continental divergence of PRRSV suggests an earlier geographic separation followed by independent evolutionary trajectories. The near simultaneous appearance of similar diseases on different continents caused by genetically disparate virus strains may reflect recent changes in swine husbandry. Specifically, whereas swine formerly were raised outdoors in relatively small groups, swine production now occurs in densely populated operations housed in confinement.
....
PRRSV is readily transmitted to susceptible sows via the semen of infected boars. Virus is shed in the semen of infected boars for as long as 43 days, and viral RNA can be detected up to approximately 100 days. Venereal infection of gilts and sows usually results only in transient anorexia, inappetence, and lethargy, but not reproductive disease, whereas subsequent spread of the virus from these animals to susceptible sows in late gestation can result in reproductive failure and sow mortality.
....
Other than porcine macrophages, PRRSV replicates in vitro only in MA-104 African green monkey kidney cells and cotton rat lung cells....The ability to grow in MA-104 cells is characteristic of North American isolates of PRRSV, whereas European strains grow extremely poorly or not at all in these cells.
....
The incidence of other infectious diseases is increased in PRRSV-infected swine hers, and mortality of up to 12 to 20% can occur. Diseases that commonly occur in PRRSV-infected swine include streptococcal meningitis, septicemic salmonellosis, Glasser’s disease (Haemophilus parasuis), exudative dermatitis, sarcoptic mange (Sarcoptes scabiei), and bacterial bronchopneumonia.’

We have already posted on sarcoptic mange in two SARS-CoV reservoirs, Demodex for Paguma and Sarcoptes for Nyctereutes:

Post # 521
 
The Wild Boar wikipage shows the range of Suis, which was introduced to the Americas. For the cholangiocarcinoma of both Chinese and African Rousettus harboring Marburg virus, the boar’s range would put it in proximity with the fruit bat along the Nile River:

Wild Boar
 
From mouse to wild boar, a report from Minnesota hypothesizes the origin of PRRSV:

’....North Carolina....’
 
How the Delta variant of SARS-CoV-2 affects the binding to human ACE2 receptors is shown in this report:

Aug 2021 Miami: Delta-Induced Changes in RBD
’....Receptor-binding beta-loop-beta motif adopts an altered but stable conformation causing separation in some of the antibody-binding epitopes.’
 
A worthwhile investigation is the introduction of wild pigs from Germany into North Carolina in 1912. Baric at Chapel Hill is Shi’s colleague, Wuhan bat lady, Zheng-li Shi, who published a shrimp virus report in 2006. Here the reader will confront a paywall/identity wall:

2006 Wuhan, China: Shi, et al. / White Spot Syndrome Of Crayfish

WSS is a baculovirus, usually found in insects.

White Spot Syndrome

As will be shown, White Spot Syndrome virus links to PRRSV via ganglioside GM1.

29 Mar 2020 57-Year-Old Shrimp Seller COVID-19 “Patient Zero”
 
Shi, et al’s WSS report, above, references Shayakmetov DM, et al (2005):

GM1 will link fenofibrate on another USMB thread as well as SARS-CoV-2 adenovirus-vectored vaccines:

2005 Seattle: Shayakmetov DM, et al / Dept. of Medicine, University of Washington School of Medicine / Ganglioside GM1
’....Deletion of penton RGD motifs affects the efficiency of both the internalization and the endosome escape of viral particles containing adenovirus serotype 5 or 35 fiber knobs....that use CD46 as a primary virus attachment receptor....GM1, a lipid-raft ganglioside that plays a critical role in binding respiratory viruses and endocytosis.’
 
Trump’s Hydroxychloroquine Treatment for SARS-CoV-2 Was Correct

Raoult’s original approach was also correct. Linking Raoult’s use of azithromycin and hydroxychloroquine is GM1:

May 2020 Marseille, France: INSERM UMR_S 1072, Synergistic Antiviral Effect of Hydroxychloroquine and Azithromycin in Combination Against SARS-CoV-2: What Molecular Dynamics Studies of Virus-Host Interactions Reveal
’....Unexpected molecular similarity is shown between ATM (azithromycin) and the sugar moiety of GM1, a lipid raft ganglioside acting as a host attachment cofactor for respiratory viruses. Due to this mimicry, ATM interacts with the ganglioside-binding domain of SARS-CoV-2 spike protein. This binding site shared by ATM and GM1 displays a conserved amino acid triad, Q-134/F-135/N-137, located at the tip of the spike protein. CLQ-OH (hydroxychloroquine) molecules are shown to saturate virus attachment sites on gangliosides in the vicinity of the primary virus receptor, angiotensin-converting enzyme 2 (ACE2)....ATM is directed against the virus, whereas CLQ-OH is directed against cellular attachment cofactors. We conclude that both drugs act as competitive inhibitors of SARS-CoV-2 attachment to the host-cell membrane.’
 
The communist virus, SARS-CoV-2’s fatal mistake was to not have mutated position 137 of the spike protein. The asparagine at that location is also the same in TGEV, a porcine coronavirus known to be able to subsist on metal surfaces for up to 28 days.
 
GD1 is a ganglioside that links adenoviruses to monoclonal antibodies and vaccines. GM1, however, not only links to hydroxychloroquine and azithromycin in the French study, it links to what may be SARS-CoV-2’s most ancient ancestor. The cells being used are a derivative line from African Green Monkey:

2011 College of Veterinary Medicine, Nanjing Agricultural University, Jiangsu, China: Cholera Toxin / Ganglioside GM1 / PRRSV
’....MARC-145 cells....incubated for 1h with 100 microL monoclonal antibody against the N protein of PRRSV at 37 degrees C....An optimum dilution (1:100) of Fluroescein (FITC)-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) (Boster Biotech Ltd., Wuhan, China) was added and incubated for 45min. At 37 deg. C.
....
In order to study whether cholesterol plays a role in PRRSV infection in MARC-145 cells, we first used MbetaCD, which is widely used to sequester cholesterol from the plasma membrane. Cholesterol depletion affects lipid-raft-dependent pathway in MARC-145 cells. Cholera toxin B (CTB) is internalized in a lipid-raft-dependent manner after binding to its receptor, GM1 ganglioside.’

This trajectory has to do with the anti-SARS-Cov-2 effect of fenofibrate and cholesterol-lowering statins, of which there is already a thread here at USMB.
 
The above study continues:

’Inhibition of cholesterol synthesis inhibits PRRSV entry. Mevinolin is another lipid rafts depletion drug and it inhibits the 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase enzyme responsible for the production of mevalonate, a precursor in cholesterol biosynthesis....Virus titers and genome copies of PRRSV on mevinolin-treated cells were reduced by 34-fold and 90%. However, no significant changes on virus attachment and post-entry stage were detected....These data suggest that PRRSV infection is likely to be mediated by a lipid-raft-dependent pathway.’
 
The target is the sampling of bat SARS-like viruses at Kunming, Yunnan Province, China, as we link lupus to rabies vaccination:

2014 Kunming / Rabies / Myd88

During the same year, a vaccine adjuvant:

2014 India / Rabies Vaccine Genetic Adjuvant

Myd88 / Lupus / Belimumab

Alabama / Belimumab Antibody Titers

GM1 was mentioned in post #690.

Hungary: Lupus / GM1 / Lipid Raft Aggregation

May 2020 Fenofibrate / Lupus

2016 India: Lupus Hypertriglyceridemia in Children / Lipoprotein Lipase Antibodies / Hydroxychloroquine

One coronavirus linked to lipoprotein lipase is mouse hepatitis virus:

Linking back to Myd88-rabies at Kunming:

Nov 2020 U. Of Utah, U. Of California / MHV /Myeloid TREM2 / Cystatin F / Insulin Growth Factor 1
 
Kunming Rabies Myd88
 
Here is linked yellow head virus of shrimp to Zheng-Li Shi’s Wuhan 2006 study of white spot syndrome of shrimp (post #686), linking Indian lupus in children and hydroxychloroquine (post # 692) to PLX5622 in the myeloid TREM2/cystatin F study in the same post:

Dec 2020 Anderson Cancer Center, Houston, Texas; Baylor University, Waco, Texas; Department of Radiation Medicine, Oregon Health and Sciences University, Portland, Oregon
PLX5622 / Toll-Like Receptor 4 / Carcinoma / Myd88

May 2021 Mexico-Thailand: Yellow Head Virus of Shrimp / Toll Signaling
 
Cowley and Walker (Australia) expound upon yellow head virus of shrimp:

’The natural distribution of Penaeus monodon and the genetic relationships of YHV (yellow head virus) complex viruses from different geographic locations suggest an association that may predate the tectonic drift that dispersed the Gondwanaland supercontinent some 260 million years ago. This is consistent with the ancient origins and evolutionary conservation of penaeid shrimp, for which Triassic fossils have been discovered in Madagascar; examples of the genus Penaeus have been reported to date to the late Cretaceous period. Thus okaviruses may be regarded as primitive time capsules that provide unique insights into aspects of nidovirus evolution.
....
A virus morphologically indistinguishable from YHV was subsequently observed in healthy wild and farmed P. monodon in eastern Australia in 1993-1994. To distinguish this virus from the highly virulent YHV reported from Thailand, it was named lymphoid organ virus (LOV).’
(Cowley JA, Walker PJ, Molecular Biology and Pathogenesis of Roniviruses, in Nidoviruses, Ch. 24)
 
Thus linking SARS-CoV-2 uridylate-specific endoribonuclease is gill-associated virus (GAV) uridylate-specific endoribonuclease:

Mar 2021 India: SARS-CoV-2 Uridylate-Specific Endoribonuclease / Potent Inhibition by Thymopentin and Oleuropein

’Subsequent nucleotide sequence analysis confirmed that YHV and GAV (gill-associated virus) are closely related but distinct viruses....Sequence alignments of the region downstream of the helicase indicate that GAV pp1b also possesses 3’-to-5’ exonuclease, uridylate-specific endoribonuclease, and C-terminal ribose-2-O-methyltransferase (2’-O-MT) motifs, which, in coronaviruses, have been shown to be essential to RNA synthesis and progeny virus production.’
(Cowley and Walker, op cit)
 
The mouse hepatitis coronavirus (MHV, post #692) links to Ralph Baric’s co-authorship with Wuhan’s Zheng-Li Shi for adenovirus-SARS-CoV constructs:

Baric RS, Sims AC (2005) Development of Mouse Hepatitis Virus and SARS-CoV Infectious cDNA Constructs, Current Topics in Microbiology and Immunology, 287:229-252.
 
Two Baric RS studies are:

2006 SARS Coronavirus Vaccine Development

2005 Development of Mouse Hepatitis Virus and SARS-CoV Infectious cDNA Constructs
 
Violence in rural Oregon, an example of the stress the Chinese communist virus is causing in America:

29 Aug 2021 Oregon: Delta-Fueled Infections
’....”I’ve been an ICU nurse for 10 years. I’ve never seen anything like this. It’s really terrifying for them, and it’s really difficult for us to see day in and day out. We admit nine unvaccinated to every one vaccinated individual.” ‘
 
A nidovirus linked to mosquitoes in Vietnam (Nam Dinh virus), we note the post-COVID-vaccine thrombotic symptom caused by a Phlebovirus:

28 Aug 2021 Japan: Tick-Borne SFTSV
‘....SFTS virus, genus Phlebovirus, family Bunyaviridae.’
 

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