Snake Meat......source of chinese virus

[Unkotare]

The fact that you reach for such broadly illogical conclusions speaks volumes about YOU.

People would be up in arms if that was happening in the US. The fact that they can commit horrifying wide spread cruelty to dogs, speaks volumes about Chinese culture. Why arent YOU critical of china too?

It’s NOT widespread. Only a very small, and rapidly shrinking, number of people in China today eat dog. Almost everyone under 30 finds it appalling. There is a large and growing movement to outlaw the annual ‘festival’ that upsets so many people there and around the world.

You mean the festival where they torture puppies? Yeah, we should never criticize that.

Can you read at all?

I knew there was no way you would be able to actually discuss a topic or defend your position. ...?

Read my response before shooting your mouth off with that bullshit, jackass.

 

[Unkotare]

Has anyone had a chance to point out just how filthy, disgusting and inhuman Chinese people are yet??

Maybe if we keep stressing this point, this may help with combating the virus...

The videos of what they do to dogs is extremely disturbing. The fact that they allow that shit to happen speaks volumes about Chinese people. That is a rutheless culture, so it deserves a lot of criticism.

The fact that you reach for such broadly illogical conclusions speaks volumes about YOU.

People would be up in arms if that was happening in the US. The fact that they can commit horrifying wide spread cruelty to dogs, speaks volumes about Chinese culture. Why arent YOU critical of china too?

It’s NOT widespread. Only a very small, and rapidly shrinking, number of people in China today eat dog. Almost everyone under 30 finds it appalling. There is a large and growing movement to outlaw the annual ‘festival’ that upsets so many people there and around the world.

^^^

 

[MacTheKnife]

Virologist: Overseas Production of Vaccines Makes U.S. Vulnerable

 

[badger2]

#203: More than vaccine manufacturing needs to return to the U.S. Go Trump!

At Avian Flu Talk, a thread arises mentioning pangolins that show almost identical to 2019-nCoV. One problem with this is that we can already see where pangolin (Manis javanica) relationship is on the chart in the study for snakes by Ji, et al. Another is that pangolins align nicely, except for the HIV and ACE2 parameters. In previous posts, the civet was pretty much excluded for ACE2.

 

[badger2]

Badger Attempts to Start a Trend in Wisconsin

The new "cowboy" look, the triangle of the bandana is placed over the Adam's Apple and can be quickly raised over the mouth and nose when sneezing, coughing, or something funky comes within range. Unfortunately, since the Chinese have recently expanded their diagnostic criteria to include conjunctivitis, transmission via the eyes is a disturbing possibility.

Atomic Wuhan

Hats off to at least two Canadians and the Pasteur Institute for the cutting-edge study in post #182. By aligning atoms, spatial and structural relationships are revealed that cannot by current conventional methods:

'South and Central American emergent influenza A viruses H17N10, isolated from bats in Guatemala, and H18N11, isolated from bats in Peru, have highly sequence divergent N10 and N11 proteins that do not process the artificial substrate methylumbelliferyl-N-acetyl-alpha-D-neuraminic acid ("MUNANA"). The N10 and N11 proteins were characterized as "neuraminidase-like" because components of a functional active site were not identified in the structural reports and the proteins showed n o activity by cleavage assays, e.g., MUNANA cleavage. No other N10 or N11 protein cell entry domains were identified in the reports of the x-ray crystal structures of these proteins....The lack of activity of N10 and N11 proteins is problematic as loss of sialidase activity, in the absence of some compensating change, would be expected to reduce the fitness of any influenza A virus that incorporates these proteins....We used the common relative spatial occupancy of atoms in N10 and N11....and functionally validated influenza A, influenza B, and bacterial neuraminidases to superpose the structures....we identified a previously unidentified site....variable loop regions in the N10 and N11 proteins that present residues forming domains associated with cell entry in non-neuraminidase proteins, such as toxins and Hepatitis E and SARS viral coat proteins. The absence of demonstrated neuraminidase activity with the presence of new cell entry domain components in N10 and N11 proteins suggest that N1- and N11-containing viruses may enter cells without a functioning sialidase, i.e., by binding to alternative receptors such as ACE2, acetylcholine, and MHC II receptors on an expanded receptive cell population, including cells such as neurons and T-cells.
....
The atoms listed in Table 1 were found to have nearly identical spatial distribution in the N10P, N11P, N6N, IBN and SPN structures....We found multiple, non-neuraminidase domains in the Upside VLRs (variable loop regions): Staphylococcal Enterotoxin I in the N10P Upside VLR; hepatitis E2S protein and SARS spike protein in the N11P Upside VLR; and substance P in the N6N Upside Vlr. We also found toxin-like domains in the N11P Downside VLRs; these toxin-like domains were present in alpha-bungarotoxin, anthrax lethal factor, clostridium botulinum neurotoxin and tetanus toxin.....The common reference orientation of E2S and N11P residues is achieved by superposing the atoms with common distributed geometry listed in Table 6. Fig. 10 shows SARSSP (spike protein) and corresponding N11P residues presented in different and common reference orientations....the loops containing residues P105-P108 in N11P and residues P469-P472 in the SARSSP are mobile. The P469-P472 residues in SARSSP could easily reposition to bind within a monomer, instead of across monomers as shown in Fig. 10.
....
As can be seen from Fig. 11, there is a strong structural correspondence between the individual N11P domains mapped onto ABT (alpha-bungarotoxin), suggesting that movement of mobile loops produces the same combined domain structure in N11P and ABT. This set of residues in other toxins suggest its importance. Table 9 lists residue correspondences between N11P, SEI, ABT, ALF, CBN, and TTX. Fig. 12 shows that these structurally characterized toxins present similar clusters of N11P Downside VLR residues on mobile loops.
....
The presence of E2S-like, SARS spike protein-like, or tozin-like domains by the N10 and N11 proteins in these emerging viruses may indicate that H17N10 and H18N11 sialidase-facilitated cell entry has been supplemented or replaced by sialidase-independent receptor binding to an expanded cell population that may include neurons and T-cells....An avian-origin pathogenic H7N9 influenza A virus has emerged in China that causes severe pneumonia and has adapted to replicate in human conducting and lower airways of humans.'
(Weininger A, Weininger S, Using Common Spatial Distributions of Atoms to Relate Functionally Divergent Influenza Virus N10 and N11 Protein Structures to Functionally Characterize Neuraminidase Structures, Toxin Cell Entry Domains, and Non-Influenza Virus Cell Entry Domains, PLos One (2015) 10(2): e0117499)

 

[badger2]

Thus, influenza viruses in bats from Guatemala and Peru (H17N10/H18N11) link the Chinese krait, Bungarus multicinctus toxin to SARS coronavirus spike protein.

 

[badger2]

From Avian Flu Talk, we hear of the first Swedish case at Jonkoping, and the 10th case in Taiwan.

The Pentagon prepares military quarantine:

The Pentagon is preparing to house up to 1,000 people at military sites who may need to be quarantined due to the Wuhan coronavirus outbreak

 

[badger2]

1 Feb 2020 U.S. Declares Public Health Emergency

 

[badger2]

Avian Flu Talk: ' 2 Feb 2020 Bird Flu Outbreak in Hunan....lies on the southern border of Hubei province....H5N1....is much deadlier than either SARS or the coronavirus....2019-nCoV: a study by Hong Kong University predicted on Friday that as many as 75,815 people have been infected.....1 Feb 2020 mainland China: 13, 801 cases, 304 deaths; Hong Kong 14 cases, Macau 7 cases Taiwan 10 cases, rest of Asia 90 cases, Europe 22 cases, North America 12 cases, Australia 12 cases.'

In the Ji et al report (previous posts), squared euclidean distance to 2019-nCoV based on relative synonymous codon usage (RSCU) shows in descending order of proximity, Homo sapiens, Gallus gallus, Rhinolophus sinicus Manis javanica, Erinaceus europaeus (European hedgehog), Marmota, Naja atra, Bungarus multicinctus.

 

[badger2]

Deadly H5N1 Bird Flu Outbreak in Hunan Province
China reports H5N1 bird flu outbreak in Hunan province

 

[badger2]

We currently do not have the Chinese demographics for this strain:

The Next Bird Flu Strain is Deadlier Than the Last

 

[badger2]

In post #204, we ruled out pangolin (Manis javanica) because HIV and ACE2 parameters were lacking in the alignments.

2 Feb 2020 Cocktail of Flu, HIV Drugs Appears to Help Fight Coronavirus: Thai Doctors
Cocktail of flu, HIV drugs appears to help fight coronavirus: Thai doctors

 

[badger2]

Statement About nCoV and Our Pandemic Exercise
https://www.centerforhealthsecurity...-24-Statement-of-Clarification-Event-201.html

 

[badger2]

Post #213 was from Avian Flu Talk and the webpage clarifies Johns Hopkins/ Bill and Melinda Gates' Event- 201, which was a mock coronavirus pandemic exercise in October of 2019.

 

[badger2]

The cocktail of the Thai doctors used oseltamivir. What is little known about this drug is its much more intimate relationship to Thailand and Laos. There is a history of heavy trade of Laotian star anise into North Vietnam, and we can even pinpoint the border location for this trade.

Lonely Planet Tai-Lao Open Border Market
https://www.lonely.com/thailand/tha...o-open-border-market/a/poi-sig/1575593/357735
'....but some Lao traders come over to sell herbs, roots, mushrooms, bats and other forest products....'

Jan 2020 Oseltamivir / UK / MERS-CoV
Middle East Respiratory Syndrome Coronavirus (MERS-CoV) - surveillance and testing in North England from 2012 to 2019. - PubMed - NCBI

Jan 2020 Star Anise / Oseltamivir
Star anise (Illicium verum): Chemical compounds, antiviral properties, and clinical relevance. - PubMed - NCBI
'....shikimic acid, which is used in the manufacture of oseltamivir (Tamiflu).'

May 2009 A Short Practical Synthesis of Oseltamivir Phosphate (Tamiflu) from (-)-Shikimic Acid
A short and practical synthesis of oseltamivir phosphate (Tamiflu) from (-)-shikimic acid. - PubMed - NCBI

 

[badger2]

NY Times Wuhan Coronavirus Looks Increasingly Like a Pandemic, Experts Say
Wuhan Coronavirus Looks Increasingly Like a Pandemic, Experts Say

We'll take a closer look at the other meds in the cocktail.

Avian Flu Talk: 'OK, here it is, the specifics, again Bloomberg: oseltamivir along with lopinavir and ritonavir, both HIV drugs....the reported use of an experimental drug from Gilead Sciences Inc, called remdesevir.'

What the pangolin sequences did not align with looks closer than anything thus far: HIV and ACE2.

 

[badger2]

Recently, we were preparing to look into VSV, basis for ebola vaccine for comparison with spikeless and temperature-sensitive coronavirus mutants. For the origins of remdesevir mentioned above, ebola virus links to coronavirus:

2016 GS-5734 (Remdesevir) / Ebola / Coronavirus
Therapeutic efficacy of the small molecule GS-5734 against Ebola virus in rhesus monkeys. - PubMed - NCBI

 

[badger2]

28 Jan 2020 Coronavirus Latest Updates: First Case Reported in Africa
Coronavirus latest updates: First case reported in Africa
'....arrived at Felix-Houphouet-Boigny International Airport, Abidjan, Ivory Coast....'

 

[badger2]

This came in about 3 minutes ago:

U.S., 2nd Case Bay Area Confirmed
https://www.nbcbayarea.com/news/local/south-bay/second-case-of-coronavirus-confirmed/12225499/

 

[badger2]

https://www.nbcbayarea.com/news/loc...e-coronavirus-in-bay-area-confirmed/12225499/