Loud and clear.
Prokaryotes do not have a nucleus.
The DNA is not enclosed by a membrane.
Eukaryotes are a relatively recent invention. Took about 2 billion years to get one.
2x10^9 * 60x60x24x365 seconds. Plus a few extra for leap years.
That's about 60x10^15 seconds. With an average prokaryote generation around 4 days, that would be approximately 700 billion generations.
Prokaryote DNA is unprotected, it doesn't have any repair mechanisms. It suffers the full force of random mutations, which are 60-70 per generation (depending on sequence length).
So, 60x700x10^9 is about 4 trillion mutations. Times 10^30 instances, which is how many prokaryotes there are on average.
So maybe 10^42, give or take a few orders of magnitude.
There are only 10^21 stars in the universe. So we're talking the number of stars squared.
Do you think we could find a nuclear membrane in there somewhere? After all, it's just a cell within a cell. One cell eats another, suddenly you have a nuclear membrane. Our cells ate mitochondria too, there's another membrane. There's membranes all over the place. Membranes are easy.