Snake Meat......source of chinese virus

[badger2]

This is what Epstein says about Micro syndrome and microcephaly:

'Warburg Micro syndrome (OMIM 60018) and Martsolf syndrome (OMIM 21270) are autosomal recessive neurodevelopmental disorders that may result from the dysregulation of the Rab3 pathway. Inactivating germline mutations....Micro syndrome is a severe disorder characterized by ocular (microphthalmos, microcornea, congenital cataracts, optic atrophy; neurodevelopmental microcephaly, cortical gyral abnormalities -- polymicrogyria, hypoplasia of the corpus callosum, severe learning disability, spastic cerebral palsy) and endrocrine abnormalities. Martsolf has an overlapping but less severe phenotype.'
(Epstein, Inborn Errors of Development, Ch. 146 RAB3GAP1 and RAB3GAP2 and Warburg Micro and Martsolf Syndromes)

 

[badger2]

As far as is known, there are no follow-up reports for the Yangtze Finless Porpoise as potential intermediate host of COVID-19, although the porpoise swims past Wuhan, most likely frequently.

COVID Origins Off-Limits

Nothing to see: COVID origins off-limits as China's Wuhan touts recovery

'....on the banks of the Yangtze river....China has shown little appetite for an international enquiry into the origins of COVID-19....'

 

[badger2]

At Avian Flu Talk (COVID forum ) a thread appears, 'Super Computer Comes Up With New COVID Theory', the link is here:
https://elemental.medium.com/a-supercomputer-analyzed covid-19-and-an-interesting-new-theory-has-emerged-31cb8eba9d63
'....the bradykinin hypothesis....COVID-19.....causes the body's mechanisms for regulating bradykinin to go haywire....'

On 17 Jul 2020 at post #756, we mentioned the bradykinin homologue
homologue in amphibians, maximakinin, from the Chinese Red Belly Toad, Bombina maxima. Interestingly in this report, one again notes the double arginines (RR, of post #989):

Snake Meat......source of chinese virus

A nurse relates her COVID experience: https://www.avianflutalk.com/from-the-frontlines_topic42821.html '....Ladies and Gentlemen, I have had the flu, I've had mumps on the pancreas, chicken pox, measles, broken neck and had total knee replacement. If you were to combine the painful effects of...

www.usmessageboard.com

'....Plummer's inhibitor (La Jolla, Ca.)....Reference #7: GDP-Locked Rab Mutants....'

We have already shown the double arginine link to La Jolla (post #989), thus linking back to Rab genes of previous posts. Shortly we will post a bit more on this amphibian bradykinin.

 

[badger2]

Here is the AFT thread:

Super Computer Comes Up With New COVID Theory

Super Computer Comes Up With New Covid Theory

I didn't even know what bradykinin was. Or that those of African American descent have up to five...

www.avianflutalk.com

 

[badger2]

The Rab/ACE/Bradykinin trajectory in amphibians continues:

D-Arg0-Bradykinin-Arg-Arg, a Latent Vasoactive Bradykinin B2 Receptor Agonist Metabolically Activated by Carboxypeptidases - PubMed

We previously reported hypotensive and vasodilator effects from C-terminally extended bradykinin (BK) sequences that behave as B₂ receptor (B₂R) agonists activated by vascular or plasma peptidases. D-Arg⁰-BK-Arg-Arg (r-BK-RR) is a novel prodrug peptide...

www.ncbi.nlm.nih.gov

'....We previously described the brief hypotensive response associated with tachycardia in response to the i.v. injection of increasing doses of bradykinin (bk) in anesthetized rats and the strong potentiation of the responses following pharmacologic ACE blockade....The lack of effect of ACE inhibitors on the apparent potency of bk has been previously reported....immunoreactive ACE is limited to the single endothelial cell layer of the luminal surface of the vein....The absolute and relative abundance of ACE and arg-CPs may not fully represent their in vivo role at the level of resistance arterioles.....These results underscore the important roles played by ACE, as the main r-bk-inactivating peptidase in the extracellular space....the active reaction product r-bk is itself cleared by ACE and in the endosomes.'

 

[badger2]

So, the supercomputer report (#1,004) states, 'ACE normally degrades bradykinin, but when the COVID-19 virus downregulates it, it can't do this as effectively.'

So too, with the Chinese toad (post #1,005): 'ACE, as the main r-bk-inactivating peptidase....r-bk itself is cleared by ACE in the endosomes.'

 

[badger2]

The lab myth will end once the source of COVID-19 in nature is found.

Aug-Sep 2020 (Paris-Marseille) Tracing the Origins

[Tracing the origins of SARS-COV-2 in coronavirus phylogenies] - PubMed

SARS-CoV-2 is a new human coronavirus (CoV), which emerged in People's Republic of China at the end of 2019 and is responsible for the global Covid-19 pandemic that caused more than 540 000 deaths in six months. Understanding the origin of this virus is an important issue and it is necessary to...

www.ncbi.nlm.nih.gov

'....On the basis of currently available data, it is impossible to determine whether SARS-CoV-2 is the result of a natural zoonotic emergence or an accidental escape from experimental strains.'

 

[badger2]

This report is from Ann Arbor and Johns Hopkins:

Aug 2020 Rethinking Gain-of-Function

Rethinking Gain-of-Function Experiments in the Context of the COVID-19 Pandemic - PubMed

Proponents of the use of gain-of-function (GOF) experiments with pathogens with pandemic potential (PPP) have argued that such experiments are necessary because they reveal important facets of pathogenesis and can be performed safely. Opponents of GOF experiments with PPP have argued that the...

www.ncbi.nlm.nih.gov

'....At this time, the scientific consensus is that the virus emerged as a zoonosis whereby it jumped from an animal host, possibly bats, or pangolins, to humans, and arguments about a laboratory origin for SARS-CoV-2 are more akin to a conspiracy theory than to a scientifically credible hypothesis. In the very unlikely event that SARS-CoV-2 had emerged by accidental escape from a lab, however, that would be a great cause for concern because the Wuhan facility was state of the art and presumably operating with a high degree of care.'

 

[badger2]

There are many important reasons to maintain this thread.

A Potential Explanation for a Distinctive Genome

Might SARS-CoV-2 Have Arisen via Serial Passage through an Animal Host or Cell Culture?: A potential explanation for much of the novel coronavirus' distinctive genome - PubMed

Despite claims from prominent scientists that SARS-CoV-2 indubitably emerged naturally, the etiology of this novel coronavirus remains a pressing and open question: Without knowing the true nature of a disease, it is impossible for clinicians to appropriately shape their care, for policy-makers...

www.ncbi.nlm.nih.gov

'....Unless the intermediate host necessary for completing a natural zoonotic jump is identified, the dual-use gain-of-function research practice of viral serial passage should be considered a viable route by which the novel coronavirus arose....and its unexpectedly high affinity for angiotensin....glycosyltransferase....'

One good reason for maintaining this thread is to dispel the tendency toward conspiracy theory virology, which can swiftly become politicized for use in the 2020 U.S. Presidential election.

 

[badger2]

Where the bradykinin trajectory mutates into a leucokinin trajectory, there is still ACE connections. Because we have linked Wuhan GILT to Baruch S. Blumberg Institute GILT in previous messages, we will take an example of naturally-occurring gain-of-function phenomenon to connect these dots via hepatitis B virus.

Aug 2020 Hepatitis B Virus Naturally Occurring Gain-of-Function

Human Hepatitis B Viral Infection Outcomes Are Linked to Naturally Occurring Variants of HLA-DOA That Have Altered Function - PubMed

HLA molecules of the MHC class II (MHCII) bind and present pathogen-derived peptides for CD4 T cell activation. Peptide loading of MHCII in the endosomes of cells is controlled by the interplay of the nonclassical MHCII molecules, HLA-DM (DM) and HLA-DO (DO). DM catalyzes peptide loading...

www.ncbi.nlm.nih.gov

'....Analysis of SNPs genetically linked the DOA*0102 common allele, a gain-of-function variant, with human hepatitis B viral persistence.....edosomes....it may traffic from the ER somewhat independent of DM as has been reported DOalphaP11 V DO variant....DPA1....'

DPA1 / COVID-19 Early Wuhan Seafood Market Patients

HLA predictions from the bronchoalveolar lavage fluid samples of five patients at the early stage of the wuhan seafood market COVID-19 outbreak - PubMed

We are in the midst of a global viral pandemic, one with no cure and a high mortality rate. The Human Leukocyte Antigen (HLA) gene complex plays a critical role in host immunity. We predicted HLA class I and II alleles from the transcriptome sequencing data prepared from the bronchoalveolar...

www.ncbi.nlm.nih.gov

'....Both DPA1*02:02 and DPB1*05:01 occur at relatively high frequency (44.8% & 31.3%, n=1490) in Han Chinese, and associations of those HLA Type II alleles with narcolepsy and Grave's disease, both autoimmune disorders have been reported in that population. Further, a GWAS study found a link between HLA-DBP1*05:01 and chronic hepatitis B in Asians, and it has been suggested that this risk allele may impact one's ability to clear viral infections. HLA also informs vaccine development.'

(to be continued)

 

[badger2]

Blumberg himself was impressed by HBV's traffic phenomenon, which includes (return [italics]) to the ER!

Continuing the DPA1 trajectory that links the kinins (leucokinin/bradykinin), there is a hydroxychloroquine connection as well, in its anti-malarial activity:

DPA1 / Plasmodium / Owl Monkey Vaccine

Characterisation and comparative analysis of MHC-DPA1 exon 2 in the owl monkey (Aotus nancymaae) - PubMed

The Aotus nancymaae (owl monkey) is an important animal model in biomedical research, particularly for the preclinical evaluation of vaccine candidates against Plasmodium falciparum and Plasmodium vivax, which require a precisely typed major histocompatibility complex. The exon 2 from A...

www.ncbi.nlm.nih.gov

DPA1 Allele / Methionine at Position 11

HLA class II factors associated with Plasmodium falciparum merozoite surface antigen allele families - PubMed

In Plasmodium falciparum malaria, certain human leukocyte antigens (HLA) and the parasite's merozoite surface antigens 1 and 2 (MSA-1, MSA-2) have been shown to influence the course of the infection. This report is on associations of distinct HLA factors with the occurrence of particular MSA...

www.ncbi.nlm.nih.gov

We next link the furin to methionine:

Furin / Methionine / Recognition Site (RQKR)

N-terminally extended FMRFamide-related peptides of Helix aspersa: processing of the precursor protein and distribution of the released peptides - PubMed

Sequencing of cDNA clones reveals a precursor protein that can be processed into 10 different hepta-FaRPs. Two of the peptides are previously undescribed and are N-terminally extended forms of-YMRFamide, making them the only methionine-containing peptides in the precursor. They are separated...

www.ncbi.nlm.nih.gov

'....FMRFamide....'

FMRFamide Cutaneous Afferent Information

Neurons with different immunoreactivity form clusters in the CNS of Helix pomatia - PubMed

The distribution of serotonin (5HT-ir), FMRF amide (FMRF a-ir), catch-relaxing-peptide (CARP-ir), dopamine (DA-ir), gamma-amino-butyric-acid (GABA-ir), and leucokinin (LK-ir) immunoreactive neurons were compared in the ganglia of Helix CNS. These neurons are not distributed randomly, but their...

www.ncbi.nlm.nih.gov

'....areas of origin of skin nerves....leucokinin (LK-ir) immunoreactive neurons.'

Next, a Pubmed search for a leucokinin-bradykinin assemblage yielded these two entries:

Separation of Peptides

Selective separations of peptides with sequence deletions, single amino acid polymorphisms, and/or epimeric centers using macrocyclic glycopeptide liquid chromatography stationary phases - PubMed

Separating closely related peptides (those differing by one or two amino acids or the chirality of a single amino acid) can be challenging using reversed-phase liquid chromatography (LC), ion-exchange LC, or using ion-pairing agents. Also, the mobile phases that give the best separations in...

www.ncbi.nlm.nih.gov

Housefly (Musca domestica) ACE

Hydrolysis of insect neuropeptides by an angiotensin-converting enzyme from the housefly, Musca domestica - PubMed

The presence in insect tissues of peptides with structural similarities to angiotensin I and to bradykinin, the two best known substrates of mammalian angiotensin-converting enzyme, has not been reported. As part of our study to identify potential substrates for insect angiotensin-converting...

www.ncbi.nlm.nih.gov

After having found the ACE connection via Pubmed searches, we obviously wanted to know about any housefly coronaviruses. A Pubmed search,'musca coronavirus' yielded these references, recalling from previous posts that TGEV can subsist on steel surfaces for as long as 28 days:

Musca / TGEV

Identification of porcine transmissible gastroenteritis virus in house flies (Musca domestica Linneaus) - PubMed

Transmissible gastroenteritis (TGE) virus was detected in house flies (Musca domestica Linneaus) by staining with specific fluorescent antibody. The flies were collected within a swine confinement facility in which TGE was enzootic. Laboratory-reared flies were infected experimentally with TGE...

www.ncbi.nlm.nih.gov

Musca / Mechanical Transmission of Turkey Coronavirus

Mechanical transmission of turkey coronavirus by domestic houseflies (Musca domestica Linnaeaus) - PubMed

Domestic houseflies (Musca domestica Linnaeaus) were examined for their ability to harbor and transmit turkey coronavirus (TCV). Laboratory-reared flies were experimentally exposed to TCV by allowing flies to imbibe an inoculum comprised of turkey embryo-propagated virus (NC95 strain). TCV was...

www.ncbi.nlm.nih.gov

 

[badger2]

A California church pleads for relief as it continues to balk at the use of common sense. It appears that two diseases are in competition with one another. The spookiness is built-in and par for the course if the URL, transcribed as found, does not function:

truepundit.com/it-must-stop-california-church-pleads-for-relief-after-county-fines-approach-60000/
'....compliance would be disobedience to our Lord's clear commands....'

 

[badger2]

7 Sep 2020 European Second Wave?

Europe’s Virus Fear Becomes Reality With New French Peak

Europe’s fears of a coronavirus resurgence are becoming reality with France hitting a new peak and infections rising in Germany and the U.K. after a summer of lax containment.

www.bloomberg.com

'....the increase has largely been attributed to younger people being infected, leading Health Secretary Matt Hancock to warn of the dangers of ignoring social-distancing rules, which could widen the spread.'

 

[badger2]

This scientist previously worked with scrub typhus:

Australia: "Identify Host Animal Carrying COVID-19 or Risk Future Outbreaks"

'Identify host animal with COVID-19 or risk future outbreaks'

Australian Museum chief scientist says a co-ordinated research effort is required to prevent plagues sweeping the globe.

smh.com.au

 

[badger2]

This supercomputer report is late. We first mentioned bradykinin in this thread on 31 Jan 2020 (post #182).

13 Sept 2020 Supercomputer / Bradykinin

A supercomputer found a promising theory about why COVID-19 cases go downhill fast. It even explains the bizarre range of symptoms.

Patients with severe cases may produce too much bradykinin, a chemical that regulates blood pressure.

www.yahoo.com

31 Jan 2020 Bradykinin

https://www.usmessageboard.com/threads/snake-meat-source-of-chinese-virus.802608/page10#post-23972541

We picked up the bradykinin trajectory again around 17 Jul 2020 (post #756).

17 Jul 2020 Bradykinin

https://www.usmessageboard.com/threads/snake-meat-source-of-chinese-virus.802608/page38#post-25100912

 

[badger2]

This cutting edge Pfizer report links three virus families: Retroviridae, Hepadnaviridae, and Flaviviridae, the latter meshing nicely with our entries for Zika Rab5 & Rab7 (previous posts):

Expansion of Pivotal COVID-19 Vaccine Trial

Pfizer and BioNTech Propose Expansion of Pivotal COVID-19 Vaccine Trial | Pfizer

Pfizer Inc. (NYSE: PFE) and BioNTech SE (NASDAQ: BNTX) announced today that they have submitted an amended protocol to the U.S. Food and Drug Administration to expand the enrollment of their Phase 3 pivotal COVID-19 vaccine trial to up to approximately 44,000 participants which also allows for...

www.pfizer.com

'....adolescents as young as 16 years of age and people with chronic, stable HIV (human immunodeficiency virus), Hepatitis C, or Hepatitis B virus infections....'

On 8 Feb 2020, we mentioned env retroviral elements that naturally integrate into the coronavirus genome:

8 Feb 2020 Env Elements Integration, post #18

Did the coronavirus start as a bio weapon

Did the Chinese Coronavirus Start as a Bioweapon Program? | The China Report Chinese sure wanted this shut down

www.usmessageboard.com

On 10 Feb 2020 we linked env to Beijing coronavirus vaccine (post #356):

Snake Meat......source of chinese virus

The report from post #340 continues, as we find some resonance with asparagine and the serine-to-leucine mutations mentioned earlier: '....Due to very limited knowledge of this novel virus, we are unable to give reasonable explanations for the significant number of amino acid substitutions...

www.usmessageboard.com

'strategies similar to those we have used have been employed to stabilize HIV envelope glycoprotein (Env) trimers in prefusion conformation.'

 

[badger2]

Thus, Pfizer's interest in HIV patients links to naturally-integrated retroviral elements into the Nidovirus genome (Coronaviruses and Arteriviruses), whilst their vaccine implicates liver tropisms for both Flavivirus (ex. HCV & Zika) and Hepadnavirus (HBV), which by default link to MHV (mouse hepatitis coronavirus).

 

[badger2]

The projection is another 12-14 months even with a vaccine:

14 Sep 2020 12-14 Months

A top disease expert is warning of 'another 12 to 14 months of a really hard road ahead of us' and says the US has no national plan to stop it

Osterholm doubled down on Dr. Anthony Fauci's warning that Americans should expect to "hunker down" this fall and winter.

news.yahoo.com

 

[badger2]

The largest singleCOVID-19 super-spreader event cost $12 billion:

https://www.aol.com/sturgis-motorcycle-rally-linked-more-225605454.html

 

[badger2]

USMB software is strange.

Anyhow, the report states, 'The 266,796 cases of C-19 linked to Sturgis represents 19% of the 1.4 million new cases in the U.S.'