No.
Nor have any of them insisted on using the bathroom of the other biological sex.
This year, we have at our school a boy who has been dressing and wearing his hair in traditionally feminine ways since 6th grade. Not blatant, but mom jeans with colorful tops and long curly hair. Two weeks ago we had wear school colors day and he came in a maroon and blue dress. We have a very masculine appearing girl who is on the football team.
The boy does not insist on using the girls room, and the girl changes in her own locker room. She's just happy she's a starter.
We have some gay students and their parent are always keen to hear that they have been bullied. They aren't. No one cares.
See the point? I doubt it.
The point is that a person can be transgender or gay without being an asshole. It takes a gaping asshole to want to make girls uncomfortable in what is supposed to be a safe place for them to change or pee away from the male gaze.