COVID-19’s Biological Politics

[badger2]

We now know the country of origin for the D-to-G mutation in comparison with COVID-19, as the above report continues:

‘L D759G was first detected at the end of Mar in Guinea and was later replaced by the triple mutant. The triple signature was first detected in Sierra Leone (end of May 2014) and was detected in more than 90% of the viruses isolated after Jun 2014.’

 

[badger2]

The CDC report to track the D-to-G mutation in bat lung Tb1.Lu, is here:

Bat Coronavirus in Brazil Related to Appalachian Ridge and Porcine Epidemic Diarrhea Viruses

Bat Coronavirus in Brazil Related to Appalachian Ridge and Porcine Epidemic Diarrhea Viruses

Coronavirus in Bats, Brazil

wwwnc.cdc.gov

 

[badger2]

Biological politics could not come at a more inopportune time for b.l.m./a.n.t.i.f.a thugs now tearing down statues in Portland, because the biological history of the D-to-G mutation of COVID-19 links to Columbus arriving in North America. We now have the location for collection of the Appalachian Ridge Coronavirus, and the pertinent bat is Perimyotis subflavus:

Evidence Supporting a Zoonotic Origin of Human Coronavirus Strain NL63

The relationship between bats and coronaviruses (CoVs) has received considerable attention since the severe acute respiratory syndrome (SARS)-like CoV was identified in the Chinese horseshoe bat (Rhinolophidae) in 2005. Since then, several bats throughout the world have been shown to shed CoV...

jvi.asm.org

‘....We used Roche 454 sequencing to determine the viral sequences present in bat fecal samples from big brown bats captured in the Saratoga National Historical Park in New York (New England CoV [NECoV] and tricolored bats from the Chesapeake and Ohio Canal National Historical Park in Maryland (Appalachian Ridge CoV strain 2 [ARCoV2]....this observation suggests that ARCoV.2 and HCOV-NL63 originated from the same ancestor, predicting that a potential cross-species transmission event occurred about the time that Columbus arrived in North America.’

 

[badger2]

With antibody technology, viruses can be thwarted before vaccines can be developed, meaning that their source in nature will matter much less than before.

12 Oct 2020 Lilly’s LY-CoV555

Eli Lilly In Gates Foundation Supply Deal For Covid-19 Antibodies

Eli Lilly & Co. has entered into an agreement with the Bill & Melinda Gates Foundation, for future supply of the US drugmaker’s therapeutic Covid-19 antibody candidates to low- and middle-income countries.The agreement to bring equitable access to potential treatments is part of the Covid-19...

finance.yahoo.com

 

[badger2]

So far, there is little on HCOV NL63 that links to the HIF-1 gene the way that it did to COVID-19, though the hypothesis is that the unusual number of American deaths have to do with indigenous virus genomes rather than politics of administrations, and also that there is a uniqueness in living in North America that differs from the Homo sapiens host in China. The financial element clings stubbornly to COVID politics:

The next big market mover, according to Wall St.

Wall Street readies investors for the next major stock market catalyst: a COVID-19 vaccine.

www.yahoo.com

‘....The vaccine represents a more important factor than the election result for the path of equities....Aside from the vaccine, therapeutic antibodies from Eli Lilly and Regeneron have demonstrated a benefit on hospitalizations/ER visits in early studies offering a potential bridge to broader availability of vaccines, a dynamic potentially under-appreciated by investors.’

 

[badger2]

Regeneron’s approach of using a double antibody to prevent resistance mutations has been mimicked by Lilly in that the latter uses LY-COV555 and LY-COV016. Not knowing the citation, a snippet, however, shows that using a single antibody can be dangerous:

‘Viral RNA sequences revealed putative LY-COV555 resistance variants in placebo and all treatment arms. The rate of resistance variants was numerically higher in treated patients (8%) versus placebo (6%).’

 

[badger2]

There is a Type of math that Johns Hopkins University will be having trouble with regarding C-19 deaths. That math links to the immune stresses caused by non-COVID-19 parameters such as the destruction and violence caused by b.l.m./a.n.t.i.f.a as the Chinese virus raged in America, the accompanying fear and terror, a destruction and blatant disregard that compounded the problem of COVID-19 economics.

75,000 More Deaths

https://www.yahoo.com/news/75-000-more-Americans-died-150018416.html

‘....Johns Hopkins University data puts the total COVID-19 death toll in the U.S. at just below 215,000.’

 

[badger2]

The reason the URL did not function was because the spelling software for the iPad has not been turned off, and will consistently capitalize in an attempt to figure out what the typist is thinking.

 

[badger2]

It's important not to rely on these iPad crutches when writing in science and medicine, akin to the pathologies of using the quote button, which crutches we have now turned off: auto-capitalization, auto-correction, check spelling, smart punctuation, and a mechanism called 'predictive,' the disablings of which give the researcher their precious time back.

 

[badger2]

#35: that may be the reason for sabotage of the Recovery trial: that it was trying to push its own medicine. What does not compute is that hydroxychloroquine does not target remdesivir's target. At least as far as we know.

 

[badger2]

wow. the president tests negative!

 

[badger2]

Briefly, a 7-month old child admitted to Slotervaart Hospital in the Netherlands, designates the NL in HCoV-NL63, the virus that links to the North American recombination event about the time of Columbus's arrival. Here we link that virus to Wuhan Institute of Virology, circa 2010. The designation 'PeSu' is the Tricolored bat, Perimyotis:

2012 Huynh, J, et al

Evidence supporting a zoonotic origin of human coronavirus strain NL63 - PubMed

The relationship between bats and coronaviruses (CoVs) has received considerable attention since the severe acute respiratory syndrome (SARS)-like CoV was identified in the Chinese horseshoe bat (Rhinolophidae) in 2005. Since then, several bats throughout the world have been shown to shed CoV...

www.ncbi.nlm.nih.gov

'....We previously used this synthetic approach (inoculating freshly filtered fecal supernatants onto a variety of cells, including PeSu-B5L lung cells) to resurrect bat CoV HKU3, the SARS-like CoV identified in the Chinese horseshoe bat, Rhinolophus, and showed that the only block to replication of this bat virus in primate cells was the180-amino-acid portion of the spike glycoprotein known as. the receptor-binding domain (RBD)....In addition, the sequence of the orthologous ACE2 receptor of tricolor bat has not been determined, but likely presents the determinants with cross-species transmission, and notably, HCoV-NL63 receptor usage was not evaluated in these studies (20567988).'

The Pubmed abstract number is for a co-authored paper from Wuhan Institute of Virology, Australian Animal Health Laboratory, and the Department of Pharmacology of the University of Minnesota:

Pubmed 20567988

Angiotensin-converting enzyme 2 (ACE2) proteins of different bat species confer variable susceptibility to SARS-CoV entry - PubMed

The discovery of SARS-like coronavirus in bats suggests that bats could be the natural reservoir of SARS-CoV. However, previous studies indicated the angiotensin-converting enzyme 2 (ACE2) protein, a known SARS-CoV receptor, from a horseshoe bat was unable to act as a functional receptor for...

www.ncbi.nlm.nih.gov

Why didn't the Wuhan Institute of Virology not determine ACE2 usage in a virus that obviously had to do with recombination events in North America?

 

[badger2]

By 2010, the Chinese would be interested in any ACE2 information, including that from HCoV-NL63. ACE2 receptor biology of NL63 would show susceptibility of non-Chinese genomes closer linked to Europe, recalling that Fauci's D614G COVID-19 mutation links to its arising almost simultaneously in Germany and China, which is the mutation that confers increased affinity for ACE2.

 

[badger2]

It does not seem that Fauci is putting all of his cards on the table:

Trump Presses Attack on Fauci

Trump presses attack on Fauci in dispute over campaign ad

The nation's leading expert in infectious diseases has asked for a video that takes his words out of context to be removed.

www.yahoo.com

'...."WHO no longer likes lockdowns"....Fauci said, "When it became clear that infection could be spread by asymptomatic carriers who don't know they're infected, that made it very clear that we had to strongly recommend masks." '

It has taken quite some time to learn about asymptomatic carriers.

2004 Guangzhou, First Military Medical University

[Epidemiological investigation of nosocomial infection of SARS in medical staff of a hospital] - PubMed

SARS patients may have no or very limited infectivity during the latent period. Infection of SARS might be concerned with the intensity and persistence of contact with the infectious sources. The pathogenesis of SARS might involve the immune status of the potential patients, and immunodeficient...

www.ncbi.nlm.nih.gov

'....All 57 persons with close contact with the 12 medical staff suffering SARS in the latent period or with one having early-stage SARS did not show any signs of SARS during one month for observation in isolation.'

 

[WarmPotato]

Dang Badger I thought this thread was bustling but its just you XD

 

[badger2]

The Huynh et al report of post #52 mentions that bat cells were immortalized, and here we show that this immortalization links our HIF-1 investigation for mask-wearing and hypoxia. Because dengue-infected brains were similar to COVID-19-infected brains at autopsy, megakaryocytes were the pivot between the two. Thus our search, 'hif-1 megakaryocytes' revealed a runx- and sparc-gene connection:

May 2016 Megakaryocytes / RUNX2 / SPARC

Cell and Signal Components of the Microenvironment of Bone Metastasis Are Affected by Hypoxia - PubMed

Bone metastatic cells release bone microenvironment proteins, such as the matricellular protein SPARC (secreted protein acidic and rich in cysteine), and share a cell signaling typical of the bone metabolism controlled by Runx2. The megakaryocytes in the bone marrow engrafted by the metastases...

www.ncbi.nlm.nih.gov

'....secreted protein acidic and rich in cysteine....RUNX2....'

A search 'runx1 covid-19' yielded a May 2020 report:
May 2020 Tripartite Combination of Candidate Pandemic Mitigation Agents

Tripartite Combination of Candidate Pandemic Mitigation Agents: Vitamin D, Quercetin, and Estradiol Manifest Properties of Medicinal Agents for Targeted Mitigation of the COVID-19 Pandemic Defined by Genomics-Guided Tracing of SARS-CoV-2 Targets in H

Genes required for SARS-CoV-2 entry into human cells, ACE2 and FURIN, were employed as baits to build genomic-guided molecular maps of upstream regulatory elements, their expression and functions in the human body, and pathophysiologically relevant cell types. Repressors and activators of the...

www.ncbi.nlm.nih.gov

The Huynh et al report states that....

'Generation of Immortalized Bat Cells....The cells were infected, as previously described, with lentivirus vectors... For cells to be immortalized, we added 1 ml of hTERT-Bmi-1-Polyprene mixture and incubated it for 3 h at 37 degrees C.'

Next, we used the combination search 'sparc bmi-1' which yields nasopharyngeal carcinoma:

2012 SPARC / Bmi-1

Secreted protein acidic and rich in cysteine (SPARC) is associated with nasopharyngeal carcinoma metastasis and poor prognosis - PubMed

SPARC expression is common in NPC patients. Our data shows that elevated SPARC expression is a potential unfavorable prognostic factor for patients with NPC.

www.ncbi.nlm.nih.gov

'....immortalized nasopharyngeal epithelial cells (NPECs) induced by Bmi-1....SPARC positively correlated with the status of distant metastasis.'

 

[badger2]

The bustling part has to do with readers.

 

[badger2]

Note that the tripartite combination report (post #56) shows that repressors of ACE2 and furin included HIF1a, and the activators of ACE2 and furin included runx1. In the now-closed snake meat thread, we had shown that furin sequences excluded pangolins as likely intermediate hosts of COVID-19.

 

[badger2]

To help answer the question of mask wearing and hypoxia-CO2, the tripartite combination report in post #56 shows that HIF1a and POU5F1 are repressors of ACE2, which would be a thumbs up for mask wearing.

 

[badger2]

In this report from Turkey, the factor protects against C-19:

(Oct 2020 Turkey) HIF Protects Against COVID-19

Hypoxia inducible factor-1 protects against COVID-19: A hypothesis - PubMed

Hypoxia inducible factor-1 protects against COVID-19: A hypothesis

www.ncbi.nlm.nih.gov

'....By the light of these findings, we suggest that HIF stabilization may improve outcomes in COVID-19 infection by decreasing ferritin, ACE2 levels and hypoxia.'