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COVID-19’s Biological Politics
- Thread starter badger2
- Start date
ClaireH
Diamond Member
Relative to the OP and recently out by Science.org: “The researchers are now trying to understand just how Delta’s R203M mutation and others in N improve the assembly of viral particles and their mRNA delivery to host cells. They will probe whether a host protein is involved. If so, targeting it with a drug could be an effective way to stall Delta’s spread.”
www.science.org
Science | AAAS
www.science.org
- May 17, 2013
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Bump. I had forgotten about this thread.
- Thread starter
- #805
The N mutation at R203 compares with vlp’s (virus-like particles) linked to the SARS-CoV-2 spike mutation that Fau Chi got backwards in his video: D614G. Because that mutation precisely increases VSV pseudovirus particle production. VSV is the virus that ebola vaccine is based upon.Relative to the OP and recently out by Science.org: “The researchers are now trying to understand just how Delta’s R203M mutation and others in N improve the assembly of viral particles and their mRNA delivery to host cells. They will probe whether a host protein is involved. If so, targeting it with a drug could be an effective way to stall Delta’s spread.”
Science | AAAS
Is this R203M mutation unique to the Delta variant? If so, this is important news.
- Thread starter
- #806
Crucifixion of the Communist Virus
We think that Pfizer’s pill is better than Merck’s pill. The dynamics are described here:
PF-07321332
’....After the tri-fluoro acetamide moiety of the compound establishes contact with the side chain of Gln189, the cyclopropyl-proline moeity occupies the central position of the binding site, establishing a series of hydrogen bonds with the backbone of His164 and Glu166 and orienting the cyclopropyl group towards the hydrophobic S2 pocket. Meanwhile, the pyrrolidone moiety is inserted in the S1 pocket, interacting with key residues of the oxyanion loop....This interaction is conserved across all possible substrate peptide crystal structures. Subsequently, the pyrrolidone moiety rearrangement also allows the reactive nitrite group to face the catalytic Lys145 making it possible to reach the final covalent bond state which cannot be described through molecular mechanics....In the final conformation, the tri-fluoro acetamide moiety is fully inserted into the S4 subpocket, establishing two additional hydrogen bonds with the backbone of the Thr190 and Glu166.’
We think that Pfizer’s pill is better than Merck’s pill. The dynamics are described here:
PF-07321332
’....After the tri-fluoro acetamide moiety of the compound establishes contact with the side chain of Gln189, the cyclopropyl-proline moeity occupies the central position of the binding site, establishing a series of hydrogen bonds with the backbone of His164 and Glu166 and orienting the cyclopropyl group towards the hydrophobic S2 pocket. Meanwhile, the pyrrolidone moiety is inserted in the S1 pocket, interacting with key residues of the oxyanion loop....This interaction is conserved across all possible substrate peptide crystal structures. Subsequently, the pyrrolidone moiety rearrangement also allows the reactive nitrite group to face the catalytic Lys145 making it possible to reach the final covalent bond state which cannot be described through molecular mechanics....In the final conformation, the tri-fluoro acetamide moiety is fully inserted into the S4 subpocket, establishing two additional hydrogen bonds with the backbone of the Thr190 and Glu166.’
- Thread starter
- #807
Supervised Molecular Dynamics (SuMD) Insights into the mechanism of action of SARS-CoV-2 main protease inhibitor PF-07321332 - PubMed
The chemical structure of PF-07321332, the first orally available Covid-19 clinical candidate, has recently been revealed by Pfizer. No information has been provided about the interaction pattern between PF-07321332 and its biomolecular counterpart, the SARS-CoV-2 main protease...
pubmed.ncbi.nlm.nih.gov
- Thread starter
- #808
While Rand Paul hammers away at Fau Chi in the videos, we review the literature for two viruses 1.) RaTG13, SARS-CoV-2’s closest relative and 2.) RsSHC014. About 200 miles separates their discovery locations. As the ill Tongguan copper miners (location 1.)) were going into the Kunming hospital (location 2.)), Fau Chi published his testimony:
26 Ap 2012 Dual Use Research of Concern
Note the date of Nov 2011 in Fau Chi’s testimony. The virus at location 2.) was collected on 18 Ap 2011. That location is the south shore of Dian Lake, Kunming.
26 Ap 2012 Dual Use Research of Concern
Note the date of Nov 2011 in Fau Chi’s testimony. The virus at location 2.) was collected on 18 Ap 2011. That location is the south shore of Dian Lake, Kunming.
- May 17, 2013
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Is this your best guess at the Gain of Fucntion combination they used here?While Rand Paul hammers away at Fau Chi in the videos, we review the literature for two viruses 1.) RaTG13, SARS-CoV-2’s closest relative and 2.) RsSHC014. About 200 miles separates their discovery locations. As the ill Tongguan copper miners (location 1.)) were going into the Kunming hospital (location 2.)), Fau Chi published his testimony:
26 Ap 2012 Dual Use Research of Concern
Note the date of Nov 2011 in Fau Chi’s testimony. The virus at location 2.) was collected on 18 Ap 2011. That location is the south shore of Dian Lake, Kunming.
- Thread starter
- #810
We have not yet excerpted what they did with the virus from location 2.), which was already a natural recombinant before Ralph Baric further experimented on it. Furthermore, there is a tryptophan (W) mutation of interest for the virus at location 2.), and we can see in their published report what they leave out of the discussion, which is noteworthy.Is this your best guess at the Gain of Fucntion combination they used here?
- Thread starter
- #811
The bat virus from the shore of Dian Lake is RsSHC014 in the report below, authors include Peter Daszak (Eco Health Alliance) and Wuhan Institute of Virology’s Zheng-li Shi. The tryptophan mutation is noted at position 442 of the spike protein. As Fau Chi says in post #808 of this thread: ‘In addition, knowledge of a particular genetic mutation or set of mutations that facilitates influenza transmission in humans may be crucial for use in global surveillance of emerging pandemic influenza viruses.’
It should be mentioned that the Chinese expressway G8511 comes within a short distance to both RaTG13 at Tongguan and to RsSHC014 at Kunming.
pubmed.ncbi.nlm.nih.gov
They mention that there are no deletions for RsSHC014, though this is misleading, as will be shown.
It should be mentioned that the Chinese expressway G8511 comes within a short distance to both RaTG13 at Tongguan and to RsSHC014 at Kunming.
Isolation and characterization of a bat SARS-like coronavirus that uses the ACE2 receptor - PubMed
The 2002-3 pandemic caused by severe acute respiratory syndrome coronavirus (SARS-CoV) was one of the most significant public health events in recent history. An ongoing outbreak of Middle East respiratory syndrome coronavirus suggests that this group of viruses remains a key threat and that...
pubmed.ncbi.nlm.nih.gov
They mention that there are no deletions for RsSHC014, though this is misleading, as will be shown.
- Thread starter
- #812
Due to what was today discovered about the RsSCH014 virus, this report should accompany the investigation:
6 Nov 2021 Alpha Variant in Dogs and Cats / Myocarditis
www.ndtv.com
6 Nov 2021 Alpha Variant in Dogs and Cats / Myocarditis
Alpha Covid Variant Detected In Dogs, Cats: Report
A new study in the Veterinary Record reveals that pets can be infected with the alpha variant of SARS-CoV-2, which was first detected in southeast England and is commonly known as the UK variant or B.1.1.7.
www.ndtv.com
- Thread starter
- #813
We can now link this mutation when comparing cats and dogs infected with SARS-CoV-2. This report for Delta R203M does not tell the reading prisoners about frameshifting, and that will also link to viruses we know that were deliberately manipulated in the lab such as RsSHC014 from the outskirts of Kunming. There is alreay a nasty Flower and Animal Market at Kunming, where buyers can see on display orchids ripped from trees in the jungle. But these virologists wanted to know something more about anthropogenic influencers of viral evolution. That is why we will compare RaTG13 from more remote locations, with RsSHC014 from urban locations.Relative to the OP and recently out by Science.org: “The researchers are now trying to understand just how Delta’s R203M mutation and others in N improve the assembly of viral particles and their mRNA delivery to host cells. They will probe whether a host protein is involved. If so, targeting it with a drug could be an effective way to stall Delta’s spread.”
Science | AAAS
Every amino acid that falls on position 203 will be un-capitalized, in comparison with SARS-CoV-2 Delta R203M mutation:
SARS-CoV-2 N
PGSSrGTSPAR
SARS-CoV N
TPGSsRGNSPAR
RaTG13 (Tongguan copper mine, G8511 Expressway) N
TPGSSrGTSPAR
RsSHC014 (Kunming, G8511 Expressway, Kunming) N
PGSsRGNSPAR
Thusfar, R (arginine) and S (serine) dominate position 203 of the nucleoprotein. However, exemplary is an insane extremism of five sequential arginines found in the N sequence of a python nidovirus.
ClaireH
Diamond Member
That thought goes through my mind frequently but you’ve labeled it better, “reading prisoners” indeed. Regardless of great effort to use primary sources, are these sources free of bias? Are the information overlords becoming so skilled as to pay off fact checkers to give them a more neutral rating? Will public information, particularly health and scientific information, ever be free of big money influence considering certain studies are immediately ousted from consideration due to funding biases?We can now link this mutation when comparing cats and dogs infected with SARS-CoV-2. This report for Delta R203M does not tell the reading prisoners about frameshifting, and that will also link to viruses we know that were deliberately manipulated in the lab such as RsSHC014 from the outskirts of Kunming. There is alreay a nasty Flower and Animal Market at Kunming, where buyers can see on display orchids ripped from trees in the jungle. But these virologists wanted to know something more about anthropogenic influencers of viral evolution. That is why we will compare RaTG13 from more remote locations, with RsSHC014 from urban locations.
Every amino acid that falls on position 203 will be un-capitalized, in comparison with SARS-CoV-2 Delta R203M mutation:
SARS-CoV-2 N
PGSSrGTSPAR
SARS-CoV N
TPGSsRGNSPAR
RaTG13 (Tongguan copper mine, G8511 Expressway) N
TPGSSrGTSPAR
RsSHC014 (Kunming, G8511 Expressway, Kunming) N
PGSsRGNSPAR
Thusfar, R (arginine) and S (serine) dominate position 203 of the nucleoprotein. However, exemplary is an insane extremism of five sequential arginines found in the N sequence of a python nidovirus.
Science | AAAS
www.science.org
Does It Pay to Do Novel Science? The Selectivity Patterns in Science Funding
Abstract. Public funding agencies aim to fund novel breakthrough research to promote the radical scientific discoveries of tomorrow. Identifying the profil
academic.oup.com
This makes for an argument in favor of automated AI. Yet even then, the self-learning process for an AI can be absolutely contaminated with “boxes” of falsehoods, making any chance of pure learning quite the challenge if even possible.
Typically, I’m not so negative. This is more about seeing the reality of the dilemma. Beyond sifting through accepted papers funded by orgs with often specific agendas, any suggestions? I’ve scoured to find the “best” fact checker available, and the following seems to be as good as it gets, at least for right now.
Who is the Least Biased News Source? Simplifying the News Bias Chart
I examined 102 news sites on the news bias chart
towardsdatascience.com
- Thread starter
- #815
Fact checkers are only as good as the knowledge base being relied on. Therefore, fact checkers have to come this way: toward scientific facts, the facts should not bend towards them.That thought goes through my mind frequently but you’ve labeled it better, “reading prisoners” indeed. Regardless of great effort to use primary sources, are these sources free of bias? Are the information overlords becoming so skilled as to pay off fact checkers to give them a more neutral rating? Will public information, particularly health and scientific information, ever be free of big money influence considering certain studies are immediately ousted from consideration due to funding biases?
Science | AAAS
Does It Pay to Do Novel Science? The Selectivity Patterns in Science Funding
Abstract. Public funding agencies aim to fund novel breakthrough research to promote the radical scientific discoveries of tomorrow. Identifying the profil
This makes for an argument in favor of automated AI. Yet even then, the self-learning process for an AI can be absolutely contaminated with “boxes” of falsehoods, making any chance of pure learning quite the challenge if even possible.
Typically, I’m not so negative. This is more about seeing the reality of the dilemma. Beyond sifting through accepted papers funded by orgs with often specific agendas, any suggestions? I’ve scoured to find the “best” fact checker available, and the following seems to be as good as it gets, at least for right now.
Who is the Least Biased News Source? Simplifying the News Bias Chart
I examined 102 news sites on the news bias chart
- Thread starter
- #816
The Ball Python is from West Africa and links to Peter Daszak’s (Eco Health Alliance) interest in African fruit bats and viruses (ebola and Marburg) Marburg now having made it from East Africa to West Africa.
Ball python (Python regius) nidovirus N (positions 20-30):
PPRQRRRRRVQ
Africa: Tsodilo Hills Python Worship and Sacrifice, 70,000 Years
www.apollon.uio.no
CDC: Uganda Python Cave
’....Egyptian fruit bats....some bats fly dozens of miles in a single night and visit other local caves....’
We next link the chron and geography to Burmese python in Yunnan and Florida:
Burmese Python (Python bivittatus)
en.wikipedia.org
’....also competes with the American alligator....Importation was banned in January 2012 by the U.S. Department of the Interior.’
Dali City, Yunnan, links the Yunnan Institute for Endemic Disease and co-authorship with Daszak’s Eco Health Alliance. Daszak is co-author for the Kunming RsSHC014 virus, and halfway between Dali and Kunming was found a 100-year-old python specimen in a cave.
Chuxiong Cave Python
Clicking on lat. and long. coordinates will retrieve maps that show Chuxiong Cave halfway between Dali City and Kunming:
en.wikipedia.org
Rf4092 also came from the same Kunming location as RsSHC014, the virus Ralph Baric would go on to genetically manipulate in a North Carolina lab. The virus also links Obama-Biden funded Duke-NUS, Singapore:
Rf4092
’....Wuhan Institute of Virology, Eco Health Alliance, Yunnan Institute for Endemic Disease (Dali City), Programme of Emerging Infectious Disease, Duke-NUS, Singapore....collected 18 Sep 2012.’
Ball python (Python regius) nidovirus N (positions 20-30):
PPRQRRRRRVQ
Africa: Tsodilo Hills Python Worship and Sacrifice, 70,000 Years
World’s oldest ritual discovered. Worshipped the python 70,000 years ago - Apollon
A new archaeological find in Botswana shows that our ancestors in Africa engaged in ritual practice 70,000 years ago — 30,000 years earlier than the oldest finds in Europe. This sensational discovery strengthens Africa’s position as the cradle of modern man.
www.apollon.uio.no
CDC: Uganda Python Cave
We next link the chron and geography to Burmese python in Yunnan and Florida:
Burmese Python (Python bivittatus)
Burmese python - Wikipedia
Dali City, Yunnan, links the Yunnan Institute for Endemic Disease and co-authorship with Daszak’s Eco Health Alliance. Daszak is co-author for the Kunming RsSHC014 virus, and halfway between Dali and Kunming was found a 100-year-old python specimen in a cave.
Chuxiong Cave Python
Clicking on lat. and long. coordinates will retrieve maps that show Chuxiong Cave halfway between Dali City and Kunming:
Chuxiong City - Wikipedia
Rf4092 also came from the same Kunming location as RsSHC014, the virus Ralph Baric would go on to genetically manipulate in a North Carolina lab. The virus also links Obama-Biden funded Duke-NUS, Singapore:
Rf4092
Bat SARS-like coronavirus isolate Rf4092, complete genome - Nucleotide - NCBI
www.ncbi.nlm.nih.gov
- Thread starter
- #817
Chinese road workers find giant python thought to be 100 years old
Two huge snakes have been found in a roadside cave in Chuxiong, south China's Yunnan Province. The larger of the two is 12 feet and 4 inches long and is thought to be more than 100 years old.
- Thread starter
- #818
It should be pointed out that the sequences in post #813 should be compared for threonine (T) and asparagine (N) in relation to the SARS-CoV-2 P.1 Brazil-Japan variant mutation K417T/N, which the CDC changed to K417T by removing the asparagine(N). There will be more comparison to these spike-related amino acids when beginning to analyze the Kunming RsSHC014 virus manipulated by Ralph Baric.
- Thread starter
- #819
From Latin nosocomialis ‘time off from work,’ this report breaks down the stats for certain disorders:
4 Nov 2021 Hospital Acquired COVID-19
khn.org
4 Nov 2021 Hospital Acquired COVID-19
Patients Went Into the Hospital for Care. After Testing Positive There for Covid, Some Never Came Out. - KFF Health News
About 21% of patients diagnosed with covid during a hospital stay died, according to data analyzed for KHN. In-hospital rates of spread varied widely and patients had no way of checking them.
khn.org
- Thread starter
- #820
A temperature studies link:
13 Oct 2021 A Cross-Sectional Analysis of Meteorological Factors and SARS-CoV-2 Transmission in 409 Cities Across 26 Countries
www.nature.com
’....A 1 deg. C increase in temperature and RH was associated with a 3% and 0.85% decrease in daily new cases....and declining again at temperatures greater than 20 deg. C....The novelty of the virus, with less than a full annual cycle of data available, makes it difficult to disentangle a seasonal signal or inter-annual trends from meteorological factors using time series models.’
There are clear differences of amounts of virus found in bats according to season. That is why collecting/testing happens in April and October.
13 Oct 2021 A Cross-Sectional Analysis of Meteorological Factors and SARS-CoV-2 Transmission in 409 Cities Across 26 Countries
A cross-sectional analysis of meteorological factors and SARS-CoV-2 transmission in 409 cities across 26 countries - Nature Communications
Possible effects of weather conditions on COVID-19 transmission are debated. Here, the authors analyse data from early in the pandemic and show that although temperature and humidity had small effects on transmission, they were far out-weighed by the effects of non-pharmaceutical interventions.
www.nature.com
There are clear differences of amounts of virus found in bats according to season. That is why collecting/testing happens in April and October.
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